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A CRISPR/Cas9 toolkit for multiplex genome editing in plants

Hui-Li Xing 0 Li Dong 0 Zhi-Ping Wang Hai-Yan Zhang Chun-Yan Han Bing Liu Xue-Chen Wang Qi-Jun Chen 0 Equal contributors State Key Laboratory of Plant Physiology and Biochemistry, College of

A Genome-Wide Association Study on Chronic HBV Infection and Its Clinical Progression in Male Han-Taiwanese

It is common to observe the clustering of chronic hepatitis B surface antigen (HBsAg) carriers in families. Intra-familial transmission of hepatitis B virus (HBV) could be the reason for the familial clustering of HBsAg carriers. Additionally, genetic and gender factors have been reported to be involved. We conducted a three-stage genome-wide association study to identify genetic...

A Geo-Aware Taxi Carrying Management System by Using Location Based Services and Zone Queuing Techniques on Internet of Things

Taxi plays a crucial role in the transportation system because of the characteristic that can be hailed conveniently. Most of the taxi drivers obtain passengers by hunting on the road or waiting in a fixed taxi queuing point; however these methods have poor performance, high vacancy rate, and several critical problems such as air pollution and foul up traffic. This study proposed...

A Hybrid Forecasting Model Based on Empirical Mode Decomposition and the Cuckoo Search Algorithm: A Case Study for Power Load

Power load forecasting always plays a considerable role in the management of a power system, as accurate forecasting provides a guarantee for the daily operation of the power grid. It has been widely demonstrated in forecasting that hybrid forecasts can improve forecast performance compared with individual forecasts. In this paper, a hybrid forecasting approach, comprising...

A Novel Prokaryotic Promoter Identified in the Genome of Some Monopartite Begomoviruses

Geminiviruses are known to exhibit both prokaryotic and eukaryotic features in their genomes, with the ability to express their genes and even replicate in bacterial cells. We have demonstrated previously the existence of unit-length single-stranded circular DNAs of Ageratum yellow vein virus (AYVV, a species in the genus Begomovirus, family Geminiviridae) in Escherichia coli...

A Novel Reaction of Peroxiredoxin 4 towards Substrates in Oxidative Protein Folding

Peroxiredoxin 4 (Prx4) is the only endoplasmic reticulum localized peroxiredoxin. It functions not only to eliminate peroxide but also to promote oxidative protein folding via oxidizing protein disulfide isomerase (PDI). In Prx4-mediated oxidative protein folding we discovered a new reaction that the sulfenic acid form of Prx4 can directly react with thiols in folding substrates...

A Pro-Inflammatory Role of C5L2 in C5a-Primed Neutrophils for ANCA-Induced Activation

Background The complement system is crucial for the development of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). In particular, C5a and its receptor on neutrophils, CD88, play a central role. The functional role of the second receptor of C5a, C5L2, remains unclear. In the current study, we investigated the role of C5L2 in C5a-primed neutrophils for ANCA...

A Review of Point Feature Based Medical Image Registration

 WangCai MengJun-Chen Wang Open Access Review First Online: 24 August 2018 421 Downloads Part of the following topical collections:overview Abstract Point features, as the basis of lines ... will experience an upcoming explosion.   Notes Authors’ Contributions Cai Meng was directed the entire trial; Shao-Ya Guan wrote the manuscript; Tian-Miao Wang and Jun-Chen Wang assisted in

A Wheat WRKY Transcription Factor TaWRKY10 Confers Tolerance to Multiple Abiotic Stresses in Transgenic Tobacco

WRKY transcription factors are reported to be involved in defense regulation, stress response and plant growth and development. However, the precise role of WRKY transcription factors in abiotic stress tolerance is not completely understood, especially in crops. In this study, we identified and cloned 10 WRKY genes from genome of wheat (Triticum aestivum L.). TaWRKY10, a gene...

A comparison of beliefs about exercise during pregnancy between Chinese and Australian pregnant women

Science , Exercise and Health , The University of Western Australia , 35 Stirling Highway, Crawley, Western Australia 6009 , Australia 2 Kym J. Guelfi and Chen Wang are co-first authors 3 School of Women's

A convenient method of aligning large DNA molecules on bare mica surfaces for atomic force microscopy

Large DNA molecules remain difficult to be imaged by atomic force microscopy (AFM) because of the tendency of aggregation. A method is described to align long DNA fibers in a single direction on unmodified mica to facilitate AFM studies. The clear background, minimal overstretching, high reproducibility and convenience of this aligning procedure make it useful for physical...

A designed peptide targeting CXCR4 displays anti-acute myelocytic leukemia activity in vitro and in vivo

100190, P. R. ChinaYanlian Yang & Chen Wang AuthorsSearch for Xiaojin Li in:Nature Research journals • PubMed • Google ScholarSearch for Hua Guo in:Nature Research journals • PubMed • Google ScholarSearch ... • Google ScholarSearch for Chen Wang in:Nature Research journals • PubMed • Google ScholarSearch for Haiyan Xu in:Nature Research journals • PubMed • Google Scholar Contributions C.W. and H.X. designed the

A high mitochondrial transport rate characterizes CNS neurons with high axonal regeneration capacity

: Romain Cartoni, Thomas L. Schwarz, Zhigang He. Methodology: Romain Cartoni. Resources: Chen Wang. Software: Gulcin Pekkurnaz. Supervision: Thomas L. Schwarz, Zhigang He. Writing ± original draft

A mixture of anatase and rutile TiO2 nanoparticles induces histamine secretion in mast cells

Background Histamine released from mast cells, through complex interactions involving the binding of IgE to FcεRI receptors and the subsequent intracellular Ca2+ signaling, can mediate many allergic/inflammatory responses. The possibility of titanium dioxide nanoparticles (TiO2 NPs), a nanomaterial pervasively used in nanotechnology and pharmaceutical industries, to directly...

A note on convergence of weighted sums of random variables

Under uniform integrability condition, some Weak Laws of large numbers are established for weighted sums of random variables generalizing results of Rohatgi, Pruitt and Khintchine. Some Strong Laws of Large Numbers are proved for weighted sums of pairwise independent random variables generalizing results of Jamison, Orey and Pruitt and Etemadi.

A novel artificial tissue simulator for endoscopic submucosal resection training – a pilot study

Background We developed a novel artificial simulator for endoscopic submucosal dissection (ESD) as a bridge between instructional videos and animal tissue training and aimed to evaluate the feasibility of using an artificial tissue model in ESD training. Methods Eight gastroenterology fellows from one medical center were enrolled in this ESD training program. Before and after the...

A panel of four genes accurately differentiates benign from malignant thyroid nodules

Background Clinicians are confronted with an increasing number of patients with thyroid nodules. Reliable preoperative diagnosis of thyroid nodules remains a challenge because of inconclusive cytological examination of fine-needle aspiration biopsies. Although molecular analysis of thyroid tissue has shown promise as a diagnostic tool in recent years, it has not been successfully...

A reverse transcriptase-related protein mediates phage resistance and polymerizes untemplated DNA in vitro

Reverse transcriptases (RTs) are RNA-dependent DNA polymerases that usually function in the replication of selfish DNAs such as retrotransposons and retroviruses. Here, we have biochemically characterized a RT-related protein, AbiK, which is required for abortive phage infection in the Gram-positive bacterium Lactococcus lactis. In vitro, AbiK does not exhibit the properties...

A systematic review and meta-analysis: Association between MGMT hypermethylation and the clinicopathological characteristics of non-small-cell lung carcinoma

Chen Wang & Shengmin YaoDepartment of Medical Oncology, The Affiliated Ganzhou Hospital of Nanchang University (Ganzhou People’s Hospital), Ganzhou, Jiangxi, 341000, ChinaYong Wang & Jiansheng ... journals • PubMed • Google Scholar Search for Chen Wang in:Nature Research journals • PubMed • Google Scholar Search for Shengmin Yao in:Nature Research journals • PubMed • Google Scholar Search for Yong

Abscisic acid regulates TSRF1-mediated resistance to Ralstonia solanacearum by modifying the expression of GCC box-containing genes in tobacco

Although recent studies have established a significant regulatory role for abscisic acid (ABA) and ethylene response factor (ERF) proteins in plant pathogen resistance, it is not clear whether and how ABA performs this role. Previously, it was reported that an ERF protein, TSRF1, activates the expression of GCC box-containing genes and significantly enhances the resistance to...