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Association between dipsacus saponin VI level and diversity of endophytic fungi in roots of Dipsacus asperoides

Dipsacus asperoides contains multiple pharmacologically active compounds. The principal are saponins. The plant can be cultivated, but it contains lower levels of bioactive compounds than the plant in the wild. It may be the reason to exploit the endophytic fungi that colonize the plant roots in order to produce bioactive compounds. However, the endophytic fungi of D. asperoides...

Biometric recognition via texture features of eye movement trajectories in a visual searching task

, Cheng Quan. Funding acquisition: Jingwei Yue. Investigation: Jiguo Xue, Cheng Quan. Project administration: Chenggang Zhang. Resources: Cheng Quan. Software: Jiguo Xue, Cheng Quan. Supervision ... : Jingwei Yue, Chenggang Zhang. Writing ± original draft: Chunyong Li. Writing ± review & editing: Chunyong Li, Jingwei Yue, Chenggang Zhang. 22 / 24 23 / 24 1. Jain AK , Flynn P , Ross AA . Handbook of

Mirror-normal difference in the late phase of mental rotation: An ERP study

Conceptualization: Cheng Quan, Chunyong Li, Jiguo Xue, Jingwei Yue, Chenggang Zhang. Data curation: Cheng Quan, Chunyong Li, Jiguo Xue, Jingwei Yue. Formal analysis: Cheng Quan. Investigation: Cheng Quan ... . Methodology: Cheng Quan. Software: Cheng Quan. Supervision: Jingwei Yue, Chenggang Zhang. Validation: Cheng Quan. Visualization: Cheng Quan, Chunyong Li. Writing ± original draft: Cheng Quan. Writing

Uncovering the cognitive processes underlying mental rotation: an eye-movement study

, 100850, ChinaJiguo Xue, Chunyong Li, Cheng Quan, Yiming Lu, Jingwei Yue & Chenggang Zhang AuthorsSearch for Jiguo Xue in:Nature Research journals • PubMed • Google Scholar Search for Chunyong Li in:Nature ... for Jingwei Yue in:Nature Research journals • PubMed • Google Scholar Search for Chenggang Zhang in:Nature Research journals • PubMed • Google Scholar Contributions Jiguo Xue (J.X.) and Chunyong Li

Antibacterial activity identification of pCM19 and pCM12 derived from hGlyrichin

BackgroundhGlyrichin is a novel human antimicrobial peptide rich in glycine. The previous study of known human antimicrobial peptides indicated that in an eligible range, the greater corresponding antibacterial activity was consisted with the shorter peptide sequence.FindingsTwo peptides named pCM19 and pCM12 were synthesized and the antibacterial activity assay results showed...

3DSNP: a database for linking human noncoding SNPs to their three-dimensional interacting genes

The vast noncoding portion of the human genome harbors a rich array of functional elements and disease-causing regulatory variants. Recent high-throughput chromosome conformation capture studies have outlined the principles of these elements interacting and regulating the expression of distal target genes through three-dimensional (3D) chromatin looping. Here we present 3DSNP, an...

Intrathecal decompression versus epidural decompression in the treatment of severe spinal cord injury in rat model: a randomized, controlled preclinical research

preclinical research Jian Zhang 0 Huili Wang 0 Chenggang Zhang Weiguang Li 0 No.1 Department of Orthopedic Surgery, Tianjin Baodi Hospital , No.8, Guangchuan Road, Baodi District, Tianjin 301800 , China

DELTA: A Distal Enhancer Locating Tool Based on AdaBoost Algorithm and Shape Features of Chromatin Modifications

Accurate identification of DNA regulatory elements becomes an urgent need in the post-genomic era. Recent genome-wide chromatin states mapping efforts revealed that DNA elements are associated with characteristic chromatin modification signatures, based on which several approaches have been developed to predict transcriptional enhancers. However, their practical application is...

Effects of fetal microwave radiation exposure on offspring behavior in mice

The recent rapid development of electronic communication techniques is resulting in a marked increase in exposure of humans to electromagnetic fields (EMFs). This has raised public concerns about the health hazards of long-term environmental EMF exposure for fetuses and children. Some studies have suggested EMF exposure in children could induce nervous system disorders. However...

A Genome-Wide mRNA Expression Profile in Caenorhabditis elegans under Prolonged Exposure to 1750MHz Radiofrequency Fields

Objective C. elegans has been used as a biomonitor for microwave-induced stress. However, the RF (radiofrequency) fields that have been used in previous studies were weak (≤1.8W/kg), and the bio-effects on C. elegans were mostly negative or ambiguous. Therefore, this study used more intense RF fields (SAR = 3W/kg) and longer time course of exposure (60h at 25°C, L1 stage through...

Molecular Subtyping of Serous Ovarian Cancer Based on Multi-omics Data

Classification of ovarian cancer by morphologic features has a limited effect on serous ovarian cancer (SOC) treatment and prognosis. Here, we proposed a new system for SOC subtyping based on the molecular categories from the Cancer Genome Atlas project. We analyzed the DNA methylation, protein, microRNA, and gene expression of 1203 samples from 599 serous ovarian cancer patients...

Microbial Community Dynamics during the Bioremediation Process of Chlorimuron-Ethyl-Contaminated Soil by Hansschlegelia sp. Strain CHL1

Long-term and excessive application of chlorimuron-ethyl has led to a series of environmental problems. Strain Hansschlegelia sp. CHL1, a highly efficient chlorimuron-ethyl degrading bacterium isolated in our previous study, was employed in the current soil bioremediation study. The residues of chlorimuron-ethyl in soils were detected, and the changes of soil microbial...

Bidirectional Crosstalk between Stress-Induced Gastric Ulcer and Depression under Chronic Stress

Stress contributes to a variety of diseases and disorders such as depression and peptic ulcer. The present study aimed to investigate the correlation between stress ulcer and depression in pathogenesis and treatment by using chronic stress depression (CSD), chronic psychological stress ulcer (CPSU) and water immersion restrain stress models in rats. Our data showed that the ulcer...

A Lasso regression model for the construction of microRNA-target regulatory networks

Motivation: MicroRNAs have recently emerged as a major class of regulatory molecules involved in a broad range of biological processes and complex diseases. Construction of miRNA-target regulatory networks can provide useful information for the study and diagnosis of complex diseases. Many sequence-based and evolutionary information-based methods have been developed to identify...

Tat Peptide-Mediated Soluble Expression of the Membrane Protein LSECtin-CRD in Escherichia coli

-hLSECtin-CRD were prepared in our laboratory. The plasmids pET28b and pET28b-Tat were provided by Dr. Chenggang Zhang from our institute. The plasmid pCDNA3.1amyc-his-hLSECtin was provided by Dr. Li Tang

MFEprimer-2.0: a fast thermodynamics-based program for checking PCR primer specificity

Evaluating the specificity of polymerase chain reaction (PCR) primers is an essential step in PCR primer design. The MFEprimer-2.0 server allows users to check primer specificity against genomic DNA and messenger RNA/complementary DNA sequence databases quickly and easily. MFEprimer-2.0 uses a k-mer index algorithm to accelerate the search process for primer binding sites and...

VizPrimer: a web server for visualized PCR primer design based on known gene structure

Summary: The visualization of gene structure plays an important role in polymerase chain reaction (PCR) primer design, especially for eukaryotic genes with a number of splice variants that users need to distinguish between via PCR. Here, we describe a visualized web server for primer design named VizPrimer. It utilizes the new information technology (IT) tools, HTML5 to display...

MFEprimer: multiple factor evaluation of the specificity of PCR primers

Summary: We developed a program named MFEprimer for evaluating the specificity of PCR primers based on multiple factors, including sequence similarity, stability at the 3 ′ -end of the primer, melting temperature, GC content and number of binding sites between the primer and DNA templates. MFEprimer can help the user to select more suitable primers before running either standard...

Reliability analysis of the Ahringer Caenorhabditis elegans RNAi feeding library: a guide for genome-wide screens

Background The Ahringer C. elegans RNAi feeding library prepared by cloning genomic DNA fragments has been widely used in genome-wide analysis of gene function. However, the library has not been thoroughly validated by direct sequencing, and there are potential errors, including: 1) mis-annotation (the clone with the retired gene name should be remapped to the actual target gene...