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15 papers found.
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Systematic protein interactome analysis of glycosaminoglycans revealed YcbS as a novel bacterial virulence factor

, TaiwanFelix Shih-Hsiang Hsiao, FX Reymond Sutandy, Guan-Da Syu, Yi-Wen Chen, Jun-Mu Lin & Chien-Sheng Chen AuthorsSearch for Felix Shih-Hsiang Hsiao in:Nature Research journals • PubMed • Google ScholarSearch ... • PubMed • Google ScholarSearch for Jun-Mu Lin in:Nature Research journals • PubMed • Google ScholarSearch for Chien-Sheng Chen in:Nature Research journals • PubMed • Google Scholar Contributions F.S.-H.H

Cost-Effective Location Management for Mobile Agents on the Internet

Academic Editor: Geoffrey A. Cranch Copyright © 2015 Chien-Sheng Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use

Cost-Effective Location Management for Mobile Agents on the Internet

Academic Editor: Geoffrey A. Cranch Copyright © 2015 Chien-Sheng Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use

Baseband Transceiver Design of a High Definition Radio FM System Using Joint Theoretical Analysis and FPGA Implementation

; Published 16 April 2014 Academic Editor: Chung-Liang Chang Copyright © 2014 Chien-Sheng Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits

Neural Network for WGDOP Approximation and Mobile Location

; Revised 17 June 2013; Accepted 17 June 2013 Academic Editor: Ker-Wei Yu Copyright © 2013 Chien-Sheng Chen et al. This is an open access article distributed under the Creative Commons Attribution License

Neural Network for WGDOP Approximation and Mobile Location

; Revised 17 June 2013; Accepted 17 June 2013 Academic Editor: Ker-Wei Yu Copyright © 2013 Chien-Sheng Chen et al. This is an open access article distributed under the Creative Commons Attribution License

Calculation of Weighted Geometric Dilution of Precision

-Sheng Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the ... of Communication Engineering, Chung-Hua University, Hsinchu, Taiwan Received 20 April 2013; Revised 28 August 2013; Accepted 3 September 2013 Academic Editor: Anyi Chen Copyright © 2013 Chien

Calculation of Weighted Geometric Dilution of Precision

-Sheng Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the ... of Communication Engineering, Chung-Hua University, Hsinchu, Taiwan Received 20 April 2013; Revised 28 August 2013; Accepted 3 September 2013 Academic Editor: Anyi Chen Copyright © 2013 Chien

YcgC represents a new protein deacetylase family in prokaryotes

Reversible lysine acetylation is one of the most important protein posttranslational modifications that plays essential roles in both prokaryotes and eukaryotes. However, only a few lysine deacetylases (KDACs) have been identified in prokaryotes, perhaps in part due to their limited sequence homology. Herein, we developed a ‘clip-chip’ strategy to enable unbiased, activity-based...

Nε−Lysine Acetylation of a Bacterial Transcription Factor Inhibits Its DNA-Binding Activity

Evidence suggesting that eukaryotes and archaea use reversible Nε-lysine (Nε-Lys) acetylation to modulate gene expression has been reported, but evidence for bacterial use of Nε-Lys acetylation for this purpose is lacking. Here, we report data in support of the notion that bacteria can control gene expression by modulating the acetylation state of transcription factors (TFs). We...

Identification of Lactoferricin B Intracellular Targets Using an Escherichia coli Proteome Chip

Lactoferricin B (LfcinB) is a well-known antimicrobial peptide. Several studies have indicated that it can inhibit bacteria by affecting intracellular activities, but the intracellular targets of this antimicrobial peptide have not been identified. Therefore, we used E. coli proteome chips to identify the intracellular target proteins of LfcinB in a high-throughput manner. We...

A Fast Universal Immobilization of Immunoglobulin G at 4°C for the Development of Array-based Immunoassays

To maintain the antibody activity and enhance performance of array-based immunoassays, protein G was used to allow a shorter duration of immunoglobulin G immobilization at 4°C, with the antibody placed in the appropriate orientation. The multiplexed detection of six pain-related message molecules (PRMMs) was used as examples for the development of array-based immunoassays...

A Sulfhydryl-Reactive Ruthenium (II) Complex and Its Conjugation to Protein G as a Universal Reagent for Fluorescent Immunoassays

To develop a fluorescent ruthenium complex for biosensing, we synthesized a novel sulfhydryl-reactive compound, 4-bromophenanthroline bis-2,2′-dipyridine Ruthenium bis (hexafluorophosphate). The synthesized Ru(II) complex was crosslinked with thiol-modified protein G to form a universal reagent for fluorescent immunoassays. The resulting Ru(II)-protein G conjugates were...

Global Identification of Prokaryotic Glycoproteins Based on an Escherichia coli Proteome Microarray

Glycosylation is one of the most abundant protein posttranslational modifications. Protein glycosylation plays important roles not only in eukaryotes but also in prokaryotes. To further understand the roles of protein glycosylation in prokaryotes, we developed a lectin binding assay to screen glycoproteins on an Escherichia coli proteome microarray containing 4,256 affinity...

Nonsteroidal Anti-Inflammatory Drugs for Wounds: Pain Relief or Excessive Scar Formation?

Chen,2 and Peng-Hui Wang4,5,8 1Department of Obstetrics and Gynecology, Yee-Zen General Hospital, Tao-Yuan county 326, Taiwan 2Graduate Institute of Systems Biology and Bioinformatics, National Central