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Insulin secretory responses of a clonal cell line of simian virus 40-transformed B cells

We have evaluated the potential of the clonal insulin-secretory cell line HIT-T15 as a model system for investigating stimulus-secretion coupling in pancreatic B cells. In contrast to other cell lines, HIT cell insulin secretion was consistently stimulated 2- to 3-fold by D-glucose. The maximally effective concentration of glucose was 10 mmol/1; between 2 and 10 mmol/l glucose...

Properties of isolated human islets of langerhans: insulin secretion, glucose oxidation and protein phosphorylation

Diabetologia Diabetologia D. E. H a r r i s o n 0 1 0 1Nuffield Department of Clinical Biochemistry and 2Nuffield Department of Surgery, John Radcliffe Hospital , Oxford , UK 1 Dr. D. E. Harrison