Advanced search    

Search: authors:"David R. Liu"

16 papers found.
Use AND, OR, NOT, +word, -word, "long phrase", (parentheses) to fine-tune your search.

Development of hRad51–Cas9 nickase fusions that mediate HDR without double-stranded breaks

In mammalian cells, double-stranded DNA breaks (DSBs) are preferentially repaired through end-joining processes that generally lead to mixtures of insertions and deletions (indels) or other rearrangements at the cleavage site. In the presence of homologous DNA, homology-directed repair (HDR) can generate specific mutations, albeit typically with modest efficiency and a low ratio...

High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases

Chas Leichner 4 Paul A. Clemons David R. Liu 3 0 Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT , Cambridge, MA 02142 , USA 1 Department of Chemistry

Author Correction: Predictable and precise template-free CRISPR editing of pathogenic variants

A. Cassa David R. Liu David K. Gifford Richard I. Sherwood 7 M A R C H 2 0 1 9 | V O L 5 6 7 | N A T U R E | E 1 - In this Article, a data processing error had a minor effect on Fig. 3e and

Targeting fidelity of adenine and cytosine base editors in mouse embryos

Base editing directly converts a target base pair into a different base pair in the genome of living cells without introducing double-stranded DNA breaks. While cytosine base editors (CBE) and adenine base editors (ABE) are used to install and correct point mutations in a wide range of organisms, the extent and distribution of off-target edits in mammalian embryos have not been...

Phage-assisted continuous evolution of proteases with altered substrate specificity

• PubMed • Google Scholar Search for David R. Liu in:Nature Research journals • PubMed • Google Scholar Contributions M.S.P. designed the research, prepared materials and performed experiments. H.A.R ... patent applications on PACE technologies and on the evolved gene products that are disclosed in this manuscript.. Corresponding author Correspondence to David R. Liu. Electronic supplementary material

Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation

 & David R. LiuBroad Institute of MIT and Harvard, Cambridge, Massachusetts 02141, USAWeixin Tang, Johnny H. Hu & David R. Liu AuthorsSearch for Weixin Tang in:Nature Research journals • PubMed • Google ... ScholarSearch for Johnny H. Hu in:Nature Research journals • PubMed • Google ScholarSearch for David R. Liu in:Nature Research journals • PubMed • Google Scholar Contributions W.T. and D.R.L. designed the

Publisher Correction: Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

. Badran David I. Bryson David R. Liu - In this Article, owing to an error during the production process, in Fig. 1a, the dark blue and light blue wedges were incorrectly labelled as ?G?C ? T?A? and ?G?C

A programmable Cas9-serine recombinase fusion protein that operates on DNA sequences in mammalian cells

We describe the development of ‘recCas9’, an RNA-programmed small serine recombinase that functions in mammalian cells. We fused a catalytically inactive dCas9 to the catalytic domain of Gin recombinase using an optimized fusion architecture. The resulting recCas9 system recombines DNA sites containing a minimal recombinase core site flanked by guide RNA-specified sequences. We...

Discovery of a mRNA mitochondrial localization element in Saccharomyces cerevisiae by nonhomologous random recombination and in vivo selection

In budding yeast, over 100 nuclear-encoded mRNAs are localized to the mitochondria. The determinants of mRNA localization to the mitochondria are not well understood, and protein factors involved in this process have not yet been identified. To reveal the sequence determinants for mitochondrial localization in a comprehensive and unbiased manner, we generated highly diversified...

Identification of eukaryotic promoter regulatory elements using nonhomologous random recombination

Jeffrey B. Doyon 0 David R. Liu 0 0 Howard Hughes Medical Institute and Department of Chemistry and Chemical Biology, Harvard University , 12 Oxford Street, Cambridge, MA 02138, USA Understanding

In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites

Gene-editing nucleases enable targeted modification of DNA sequences in living cells, thereby facilitating efficient knockout and precise editing of endogenous loci. Engineered nucleases also have the potential to introduce mutations at off-target sites of action. Such unintended alterations can confound interpretation of experiments and can have implications for development of...

Translating DNA into Synthetic Molecules

The unique ability of nucleic acids to replicate has recently been combined with the power of combinatorial chemistry, providing a new approach to the science of drug design.

The Behaviour of 5-Hydroxymethylcytosine in Bisulfite Sequencing

David R. Liu 0 Anjana Rao 0 Jun Liu, Johns Hopkins School of Medicine, United States of America 0 1 Department of Pathology, Harvard Medical School and Immune Disease Institute, Boston, Massachusetts

Inhibition of Bacterial Conjugation by Phage M13 and Its Protein g3p: Quantitative Analysis and Model

Conjugation is the main mode of horizontal gene transfer that spreads antibiotic resistance among bacteria. Strategies for inhibiting conjugation may be useful for preserving the effectiveness of antibiotics and preventing the emergence of bacterial strains with multiple resistances. Filamentous bacteriophages were first observed to inhibit conjugation several decades ago. Here...

An in vivo selection system for homing endonuclease activity

Homing endonucleases are enzymes that catalyze the highly sequence-specific cleavage of DNA. We have developed an in vivo selection in Escherichia coli that links cell survival with homing endonuclease-mediated DNA cleavage activity and sequence specificity. Using this selection, wild-type and mutant variants of three homing endonucleases were characterized without requiring...