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Very Long Term Stability of Mixed Chimerism after Allogeneic Hematopoietic Stem Cell Transplantation in Patients with Hematologic Malignancies

The objective of this study is to analyze the evolution of chimerism of all patients transplanted for hematologic malignancies in our unit during a 20-year period, alive without relapse at 1 year after allogeneic hematopoietic stem cell transplantation (HSCT). Chimerism was tested using short tandem repeat polymorphisms after separation into mononuclear cells and granulocytes by...

Very Long Term Stability of Mixed Chimerism after Allogeneic Hematopoietic Stem Cell Transplantation in Patients with Hematologic Malignancies

The objective of this study is to analyze the evolution of chimerism of all patients transplanted for hematologic malignancies in our unit during a 20-year period, alive without relapse at 1 year after allogeneic hematopoietic stem cell transplantation (HSCT). Chimerism was tested using short tandem repeat polymorphisms after separation into mononuclear cells and granulocytes by...

Comparing Two Types of Rabbit ATG prior to Reduced Intensity Conditioning Allogeneic Hematopoietic SCT for Hematologic Malignancies

, Michael Bernimoulin, Saadia Huguet, Alessandro Casini, Thomas Matthes, Kaveh Samii, and Eddy Roosnek contributed data and reviewed the paper. Sandra Paiano, Jakob R. Passweg, and Yves Chalandon analyzed the ... data. Sandra Paiano, Jakob R. Passweg, Eddy Roosnek, and Yves Chalandon wrote the paper. Acknowledgments The authors acknowledge the contribution of the medical and nursing staff of the 5FL+ ward of the

Comparing Two Types of Rabbit ATG prior to Reduced Intensity Conditioning Allogeneic Hematopoietic SCT for Hematologic Malignancies

, Michael Bernimoulin, Saadia Huguet, Alessandro Casini, Thomas Matthes, Kaveh Samii, and Eddy Roosnek contributed data and reviewed the paper. Sandra Paiano, Jakob R. Passweg, and Yves Chalandon analyzed the ... data. Sandra Paiano, Jakob R. Passweg, Eddy Roosnek, and Yves Chalandon wrote the paper. Acknowledgments The authors acknowledge the contribution of the medical and nursing staff of the 5FL+ ward of the

Validation of SYBR Green based quantification assay for the detection of human Torque Teno virus titers from plasma

Background Quantification of titers of ubiquitous viruses such as Torque teno virus (TTV) that do not cause clinical symptoms might be helpful in assessing the immune status of an individual. We hereby describe the validation of a SYBR Green-based TTV quantification method for plasma samples. Methods Plasmids with TTV specific inserts were used for preparing standards and...

T Lymphocytes Promote the Antiviral and Inflammatory Responses of Airway Epithelial Cells

Hypothesis T cells modulate the antiviral and inflammatory responses of airway epithelial cells to human rhinoviruses (HRV). Methods Differentiated primary human nasal epithelial cells (HNEC) grown on collagen-coated filters were exposed apically to HRV14 for 6 h, washed thoroughly and co-cultured with anti-CD3/CD28 activated T cells added in the basolateral compartment for 40 h...

BAFF production by antigen‐presenting cells provides T cell co‐stimulation

The B cell‐activating factor from the tumor necrosis factor family (BAFF) is an important regulator of B cell immunity. Recently, we demonstrated that recombinant BAFF also provides a co‐stimulatory signal to T cells. Here, we studied expression of BAFF in peripheral blood leukocytes and correlated this expression with BAFF T cell co‐stimulatory function. BAFF is produced by...