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Genetic engineering in nonhuman primates for human disease modeling

, Kawasaki-ku, Kawasaki-shi 210-0821 , Japan 1 Erika Sasaki 2 Advanced Research Center, Keio University , Shinjuku-ku, Tokyo 160-8582 , Japan Nonhuman primate (NHP) experimental models have contributed

Single cell transcriptome analysis of human, marmoset and mouse embryos reveals common and divergent features of preimplantation development

The mouse embryo is the canonical model for mammalian preimplantation development. Recent advances in single cell profiling allow detailed analysis of embryogenesis in other eutherian species, including human, to distinguish conserved from divergent regulatory programs and signalling pathways in the rodent paradigm. Here, we identify and compare transcriptional features of human...

Two-photon imaging of neuronal activity in motor cortex of marmosets during upper-limb movement tasks

• Google Scholar Search for Hiroaki Mizukami in:Nature Research journals • PubMed • Google Scholar Search for Atsushi Nambu in:Nature Research journals • PubMed • Google Scholar Search for Erika Sasaki

Generation of transgenic marmosets expressing genetically encoded calcium indicators

Chronic monitoring of neuronal activity in the living brain with optical imaging techniques became feasible owing to the continued development of genetically encoded calcium indicators (GECIs). Here we report for the first time the successful generation of transgenic marmosets (Callithrix jacchus), an important nonhuman primate model in neurophysiological research, which were...

The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect distinct genomic features

Salinas-Riester Sigrid Fuchs Erika Sasaki R?diger Behr OPEN Embryonic stem cells (ESCs) are useful for the study of embryonic development. However, since research on naturally conceived human embryos is ... currently published set of marmoset ES cell lines was completed in 2009 when we published an additional marmoset ES cell line which was originally also generated by Erika Sasaki and colleagues7. Unfortunately

Resequencing of the common marmoset genome improves genome assemblies and gene-coding sequence analysis

Atsushi Iriki in:Nature Research journals • PubMed • Google ScholarSearch for Erika Sasaki in:Nature Research journals • PubMed • Google ScholarSearch for Hideyuki Okano in:Nature Research journals • PubMed

Non-Viral Generation of Marmoset Monkey iPS Cells by a Six-Factor-in-One-Vector Approach

Groundbreaking studies showed that differentiated somatic cells of mouse and human origin could be reverted to a stable pluripotent state by the ectopic expression of only four proteins. The resulting pluripotent cells, called induced pluripotent stem (iPS) cells, could be an alternative to embryonic stem cells, which are under continuous ethical debate. Hence, iPS cell-derived...

Reprogramming non-human primate somatic cells into functional neuronal cells by defined factors

Yuzaki 0 Hirotaka James Okano Erika Sasaki 0 Hideyuki Okano 0 0 Department of Physiology, Keio University School of Medicine , 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582 , Japan Background: The common

Qualitative De Novo Analysis of Full Length cDNA and Quantitative Analysis of Gene Expression for Common Marmoset (Callithrix jacchus) Transcriptomes Using Parallel Long-Read Technology and Short-Read Sequencing

The common marmoset (Callithrix jacchus) is a non-human primate that could prove useful as human pharmacokinetic and biomedical research models. The cytochromes P450 (P450s) are a superfamily of enzymes that have critical roles in drug metabolism and disposition via monooxygenation of a broad range of xenobiotics; however, information on some marmoset P450s is currently limited...

A Versatile Technique for the In Vivo Imaging of Human Tumor Xenografts Using Near-Infrared Fluorochrome-Conjugated Macromolecule Probes

Here, we present a versatile method for detecting human tumor xenografts in vivo, based on the enhanced permeability and retention (EPR) effect, using near-infrared (NIR) fluorochrome-conjugated macromolecule probes. Bovine serum albumin (BSA) and two immunoglobulins—an anti-human leukocyte antigen (HLA) monoclonal antibody and isotype control IgG2a—were labeled with XenoLight...

Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body

Azami Junko Okahara Shinichiro Nakamura Yoshitaka Hayashi Seiji Hitoshi Yasushi Itoh Takeshi Imamura Masaki Nishimura Ikuo Tooyama Hiroyuki Miyoshi Mitinori Saitou Kazumasa Ogasawara Erika Sasaki

Common marmoset CD117+ hematopoietic cells possess multipotency

Analysis of the hematopoiesis of non-human primates is important to clarify the evolution of primate-specific hematopoiesis and immune regulation. However, the engraftment and development of the primate hematopoietic system are well-documented only in humans and are not clear in non-human primates. Callithrix jacchus (common marmoset, CM) is a New World monkey with a high rate of...

Birth of Healthy Offspring following ICSI in In Vitro-Matured Common Marmoset (Callithrix jacchus) Oocytes

Intracytoplasmic sperm injection (ICSI), an important method used to treat male subfertility, is applied in the transgenic technology of sperm-mediated gene transfer. However, no study has described successful generation of offspring using ICSI in the common marmoset, a small non-human primate used as a model for biomedical translational research. In this study, we investigated...

Efficient Derivation of Multipotent Neural Stem/Progenitor Cells from Non-Human Primate Embryonic Stem Cells

The common marmoset (Callithrix jacchus) is a small New World primate that has been used as a non-human primate model for various biomedical studies. We previously demonstrated that transplantation of neural stem/progenitor cells (NS/PCs) derived from mouse and human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) promote functional locomotor recovery of...

A novel embryonic stem cell line derived from the common marmoset monkey (Callithrix jacchus) exhibiting germ cell-like characteristics

BACKGROUND Embryonic stem cells (ESC) hold great promise for the treatment of degenerative diseases. However, before clinical application of ESC in cell replacement therapy can be achieved, the safety and feasibility must be extensively tested in animal models. The common marmoset monkey (Callithrix jacchus) is a useful preclinical non-human primate model due to its physiological...

Abundant Occurrence of Basal Radial Glia in the Subventricular Zone of Embryonic Neocortex of a Lissencephalic Primate, the Common Marmoset Callithrix jacchus

Subventricular zone (SVZ) progenitors are a hallmark of the developing neocortex. Recent studies described a novel type of SVZ progenitor that retains a basal process at mitosis, sustains expression of radial glial markers, and is capable of self-renewal. These progenitors, referred to here as basal radial glia (bRG), occur at high relative abundance in the SVZ of gyrencephalic...

Double expression of CD34 and CD117 on bone marrow progenitors is a hallmark of the development of functional mast cell of Callithrix jacchus (common marmoset)

Mast cells (MCs) are developed from hematopoietic progenitor cells and play an important role in inflammation. Study of the kinetics of development and accumulation of primate MC in vivo is crucial for the control of human inflammatory diseases, as evolution of the immune system is quite rapid and inflammation including MC response is considered to be different between mouse and...

Aged vervet monkeys developing transthyretin amyloidosis with the human disease-causing Ile122 allele: a valid pathological model of the human disease

Mutant forms of transthyretin (TTR) cause the most common type of autosomal-dominant hereditary systemic amyloidosis. In addition, wild-type TTR causes senile systemic amyloidosis, a sporadic disease seen in the elderly. Although spontaneous development of TTR amyloidosis had not been reported in animals other than humans, we recently determined that two aged vervet monkeys...