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1H and 13C NMR spectroscopic studies of the ferriheme resonances of three low-spin complexes of wild-type nitrophorin 2 and nitrophorin 2(V24E) as a function of pH

The ferriheme resonances of the low-spin (S = 1/2) complexes of wild-type (wt) nitrophorin 2 (NP2) and its heme pocket mutant NP2(V24E) with imidazole (ImH), histamine (Hm), and cyanide (CN−) as the sixth ligand have been investigated by NMR spectroscopy as a function of pH (4.0–7.5). For the three wt NP2 complexes, the ratio of the two possible heme orientational isomers, A and...

45S rDNA Regions Are Chromosome Fragile Sites Expressed as Gaps In Vitro on Metaphase Chromosomes of Root-Tip Meristematic Cells in Lolium spp

BackgroundIn humans, chromosome fragile sites are regions that are especially prone to forming non-staining gaps, constrictions or breaks in one or both of the chromatids on metaphase chromosomes either spontaneously or following partial inhibition of DNA synthesis and have been well identified. So far, no plant chromosome fragile sites similar to those in human chromosomes have...

A Challenge for the Seed Mixture Refuge Strategy in Bt Maize: Impact of Cross-Pollination on an Ear-Feeding Pest, Corn Earworm

To counter the threat of insect resistance, Bacillus thuringiensis (Bt) maize growers in the U.S. are required to plant structured non-Bt maize refuges. Concerns with refuge compliance led to the introduction of seed mixtures, also called RIB (refuge-in-the-bag), as an alternative approach for implementing refuge for Bt maize products in the U.S. Maize Belt. A major concern in...

A Comparison of Loop-Mediated Isothermal Amplification (LAMP) with Other Surveillance Tools for Echinococcus granulosus Diagnosis in Canine Definitive Hosts

Background Cystic echinococcosis is highly prevalent in northwest China. A cost-effective, easy to operate diagnostic tool with high sensitivity and specificity would greatly facilitate the monitoring of Echinococcus infections in canine definitive hosts. Methods The primers used in the LAMP assay were based on the mitochondrial nad5 gene of E. granulosus sensu stricto (E...

A Comparison of Visual Assessment and Automated Digital Image Analysis of Ki67 Labeling Index in Breast Cancer

Background Ki67 labeling index (LI) is critical for treatment options and prognosis evaluation in breast cancer. Visual assessment (VA) is widely used to assess Ki67 LI, but has some limitations. In this study, we compared the consistency between VA and automated digital image analysis (DIA) of Ki67 LI in breast cancer, and to evaluate the application value of DIA in Ki67 LI...

A Finite and Instantaneous Screw Based Approach for Topology Design and Kinematic Analysis of 5-Axis Parallel Kinematic Machines

/static/dynamic modeling, analysis and optimal design of parallel mechanisms under framework of screw theory, orthopedics medical robot. Tel: +86‑22‑87402015; E‑mail: . Shuo‑Fei Yang, born in 1988, is

A Hypomagnetic Field Aggravates Bone Loss Induced by Hindlimb Unloading in Rat Femurs

A hypomagnetic field is an extremely weak magnetic field—it is considerably weaker than the geomagnetic field. In deep-space exploration missions, such as those involving extended stays on the moon and interplanetary travel, astronauts will experience abnormal space environments involving hypomagnetic fields and microgravity. It is known that microgravity in space causes bone...

A Line-Search-Based Partial Proximal Alternating Directions Method for Separable Convex Optimization

We propose an appealing line-search-based partial proximal alternating directions (LSPPAD) method for solving a class of separable convex optimization problems. These problems under consideration are common in practice. The proposed method solves two subproblems at each iteration: one is solved by a proximal point method, while the proximal term is absent from the other. Both...

A New Control Method for Second-Order Multiple Models Control System Based on Global Sliding Mode

This paper proposed a finite time convergence global sliding mode control scheme for the second-order multiple models control system. Firstly, the global sliding surface without reaching law for a single model control system is designed and the tracking error finite time convergence and global stability are proved. Secondly, we generalize the above scheme to the second-order...

A hybrid splitting method for smoothing Tikhonov regularization problem

In this paper, a hybrid splitting method is proposed for solving a smoothing Tikhonov regularization problem. At each iteration, the proposed method solves three subproblems. First of all, two subproblems are solved in a parallel fashion, and the multiplier associated to these two block variables is updated in a rapid sequence. Then the third subproblem is solved in the sense of...

A new coumarin derivative, IMM-H004, attenuates okadaic acid-induced spatial memory impairment in rats

; Xiu-yun SONG and Yingying WANG performed the main experiments; Xiu-yun SONG and Peng-fei YANG analyzed the data and wrote the paper; Wei ZUO, Lian-Kun SONG and Shuai ZHANG assisted in the experiments

A numerical simulation of metallic cylindrical sandwich shells subjected to air blast loading

The dynamic response of cylindrical sandwich shells with aluminum foam cores subjected to air blast loading was investigated numerically in this paper. According to KNR theory, the nonlinear compressibility of the air and finite shock conditions were taken into account in the finite element model. Numerical simulation results show that the compression strain, which plays a key...

A study of aortic dimension in type B aortic dissection

Difference between arch diameter and true lumen diameter in the descending aorta was studied in patients with type B aortic dissection. The diameters of the aortic arch (Proximal ϕ) and mid-descending aorta (Distal ϕ) were measured on computer tomography angiography (CTA) in 20 healthy adults. Forty-two patients with type B aortic dissection who underwent endovascular repair were...

An Empirical Study of Parameter Estimation for Stated Preference Experimental Design

; Published 31 August 2014 Academic Editor: Huimin Niu Copyright © 2014 Fei Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted

An Interobserver Reproducibility Analysis of Ki67 Visual Assessment in Breast Cancer

Background Ki67 labeling index (LI) is used as a predictive marker and is associated with prognosis in breast cancer. However, standardised methodologies for measurement are lacking which has limited its application in clinical practice. In this study, we evaluated the interobserver concordance of visual assessment of Ki67 LI in breast cancer. Methods Ki67- immunostained slides...

Application of Minimum Reward Risk Model in Reservoir Generation Scheduling

Discrete time Markov decision process is studied and the minimum reward risk model is established for the reservoir long-term generation optimization. Different form the commonly used optimization criterion of best expected reward in reservoir scheduling, the probability that the expected generation of the whole period not exceeding the predete reward target to be smallest is...

Application of functionalized nanofluid in thermosyphon

Xue-Fei Yang 0 Zhen-Hua Liu 0 0 School of Mechanical Engineering, Shanghai Jiaotong University , Shanghai 200240, People's Republic of China A water-based functionalized nanofluid was made by

Arg-Gly-Asp (RGD)-Modified E1A/E1B Double Mutant Adenovirus Enhances Antitumor Activity in Prostate Cancer Cells In Vitro and in Mice

CAR is a transmembrane protein that is expressed in various epithelial and endothelial cells. CAR mediates adenoviral infection, as well as adenovirus-mediated oncolysis of AxdAdB-3, an E1A/E1B double-restricted oncolytic adenovirus, in prostate cancer cells. This study further assessed the therapeutic efficacy of AxdAdB-3 with Arg-Gly-Asp (RGD)-fiber modification (AxdAdB3-F/RGD...

Assessing the oseltamivir-induced resistance risk and implications for influenza infection control strategies

Assessing the oseltamivir-induced resistance risk and implications for influenza infection control strategies Nan-Hung Hsieh,1 Yi-Jun Lin,2 Ying-Fei Yang,2 Chung-Min Liao2 1Department of Veterinary

Association of Intron Loss with High Mutation Rate in Arabidopsis: Implications for Genome Size Evolution

Yu-Fei Yang 0 Tao Zhu 0 Deng-Ke Niu 0 0 MOE Key Laboratory for Biodiversity Science and Ecological Engineering, and Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life