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Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation

the Nippon Food Packer, K. K. Shikoku (Tokushima, Japan), for supplying the pig ovaries. Author Contributions Conceptualization: Fuminori Tanihara, Maki Hirata, Takeshige Otoi. Data curation ... : Fuminori Tanihara, Maki Hirata, Tatsuya Takemoto, Michiko Nakai, Daiichiro Fuchimoto, Takeshige Otoi. Formal analysis: Fuminori Tanihara, Maki Hirata, Dai-ichiro Fuchimoto, Takeshige Otoi. Funding

Generation of Live Piglets from Cryopreserved Oocytes for the First Time Using a Defined System for In Vitro Embryo Production

We report the successful piglet production from cryopreserved oocytes for the first time by using a simple, high capacity vitrification protocol for preservation and a defined system for in vitro embryo production. Immature cumulus-oocyte complexes (COCs) from prepubertal gilts were vitrified in microdrops and stored in liquid nitrogen. After warming, COCs were subjected to in...

Generation of Live Piglets for the First Time Using Sperm Retrieved from Immature Testicular Tissue Cryopreserved and Grafted into Nude Mice

Cryopreservation of immature testicular tissues is essential for increasing the possibilities of offspring generation by testicular xenografting for agricultural or medical purposes. However, successful production of offspring from the sperm involved has never been reported previously. In the present study, therefore, using intracytoplasmic sperm injection (ICSI), we examined...