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A Water-Rock Coupled Model for Fault Water Inrush: A Case Study in Xiaochang Coal Mine, China

Water inrush disasters in mining frequently occur under the influence of confined water-bearing fault zones. Therefore, investigating the fault water inrush mechanism is necessary to reduce the number of occurrences of this type of disaster. In fault zones, the rock is highly fractured, and the mechanism of water conduction is complex. In this research, the seepage mechanism of...

Totipotency in the mouse

In mammals, the unicellular zygote starts the process of embryogenesis and differentiates into all types of somatic cells, including both fetal and extraembryonic lineages—in a highly organized manner to eventually give rise to an entire multicellular organism comprising more than 200 different tissue types. This feature is referred to as totipotency. Upon fertilization, oocyte...

Enhanced OCT4 transcriptional activity substitutes for exogenous SOX2 in cellular reprogramming

Molecular Biomedicine, Röntgenstraße 20, 48149 Münster, GermanyAdele G. Marthaler, Kenjiro Adachi, Guangming Wu, Davood Sabour, Sergiy Velychko, Hans R. Schöler & Natalia TapiaMedical Faculty, Heinrich Heine ... in:Nature Research journals • PubMed • Google ScholarSearch for Ulf Tiemann in:Nature Research journals • PubMed • Google ScholarSearch for Guangming Wu in:Nature Research journals • PubMed • Google

Reprogramming to Pluripotency through a Somatic Stem Cell Intermediate

Transcription factor-based reprogramming can lead to the successful switching of cell fates. We have recently reported that mouse embryonic fibroblasts (MEFs) can be directly reprogrammed into induced neural stem cells (iNSCs) after the forced expression of Brn4, Sox2, Klf4, and Myc. Here, we tested whether iNSCs could be further reprogrammed into induced pluripotent stem cells...

Germ Cell Nuclear Factor Regulates Gametogenesis in Developing Gonads

Kinarm Ko Natalia Tapia Marcos J. Arau zo-Bravo Luca Gentile Boris Greber Karin Hu bner Vittorio Sebastiano Guangming Wu Hans R. Scho ler Austin J. Cooney Qiang Wu, National University of Singapore

Zfp296 Is a Novel, Pluripotent-Specific Reprogramming Factor

Expression of the four transcription factors Oct4, Sox2, Klf4, and c-Myc (OSKM) is sufficient to reprogram somatic cells into induced pluripotent stem (iPSCs). However, this process is slow and inefficient compared with the fusion of somatic cells with embryonic stem cells (ESCs), indicating that ESCs express additional factors that can enhance the efficiency of reprogramming. We...

Derivation and Expansion Using Only Small Molecules of Human Neural Progenitors for Neurodegenerative Disease Modeling

Phenotypic drug discovery requires billions of cells for high-throughput screening (HTS) campaigns. Because up to several million different small molecules will be tested in a single HTS campaign, even small variability within the cell populations for screening could easily invalidate an entire campaign. Neurodegenerative assays are particularly challenging because neurons are...

Initiation of trophectoderm lineage specification in mouse embryos is independent of Cdx2

Guangming Wu Luca Gentile Takuya Fuchikami Julien Sutter Katherina Psathaki Telma C. Esteves Marcos J. Arazo-Bravo Claudia Ortmeier Gaby Verberk Kuniya Abe Hans R. Schler SUMMARY The separation of

Generation of Healthy Mice from Gene-Corrected Disease-Specific Induced Pluripotent Stem Cells

Using the murine model of tyrosinemia type 1 (fumarylacetoacetate hydrolase [FAH] deficiency; FAH−/− mice) as a paradigm for orphan disorders, such as hereditary metabolic liver diseases, we evaluated fibroblast-derived FAH−/−-induced pluripotent stem cells (iPS cells) as targets for gene correction in combination with the tetraploid embryo complementation method. First, after...