Advanced search    

Search: authors:"Hans R. Schöler"

36 papers found.
Use AND, OR, NOT, +word, -word, "long phrase", (parentheses) to fine-tune your search.

Totipotency in the mouse

In mammals, the unicellular zygote starts the process of embryogenesis and differentiates into all types of somatic cells, including both fetal and extraembryonic lineages—in a highly organized manner to eventually give rise to an entire multicellular organism comprising more than 200 different tissue types. This feature is referred to as totipotency. Upon fertilization, oocyte...

Transcriptional regulation of endothelial cell behavior during sprouting angiogenesis

-Woo Jeong, JungMo Kim, Susanne Adams, Hans R. Schöler & Ralf H. AdamsLaboratory of Regulatory Genomics, Max-Planck-Institute for Molecular Biomedicine, Röntgenstrasse 20, 48149, Münster, GermanyBenjamín ... Kim, Juyong Yoon & Hans R. Schöler AuthorsSearch for Hyun-Woo Jeong in:Nature Research journals • PubMed • Google Scholar Search for Benjamín Hernández-Rodríguez in:Nature Research journals • PubMed

Genome-wide tracking of dCas9-methyltransferase footprints

In normal mammalian development cytosine methylation is essential and is directed to specific regions of the genome. Despite notable advances through mapping its genome-wide distribution, studying the direct contribution of DNA methylation to gene and genome regulation has been limited by the lack of tools for its precise manipulation. Thus, combining the targeting capability of...

Nfat/calcineurin signaling promotes oligodendrocyte differentiation and myelination by transcription factor network tuning

Oligodendrocytes produce myelin for rapid transmission and saltatory conduction of action potentials in the vertebrate central nervous system. Activation of the myelination program requires several transcription factors including Sox10, Olig2, and Nkx2.2. Functional interactions among them are poorly understood and important components of the regulatory network are still unknown...

Astrocyte pathology in a human neural stem cell model of frontotemporal dementia caused by mutant TAU protein

Astroglial pathology is seen in various neurodegenerative diseases including frontotemporal dementia (FTD), which can be caused by mutations in the gene encoding the microtubule-associated protein TAU (MAPT). Here, we applied a stem cell model of FTD to examine if FTD astrocytes carry an intrinsic propensity to degeneration and to determine if they can induce non-cell-autonomous...

Factor-Reduced Human Induced Pluripotent Stem Cells Efficiently Differentiate into Neurons Independent of the Number of Reprogramming Factors

Srimasorn,1 Holm Zaehres,5 Peter Reinhardt,5 Hans R. Schöler,5 and Alexander Storch1,2,3,6 1Division for Neurodegenerative Diseases, Department of Neurology, Technische Universität Dresden, Dresden, Germany ... the paper. Hans R. Schöler contributed to provision of study material, critical revision of the paper, and fund raising. Alexander Storch contributed to conception and design, data analysis and

Factor-Reduced Human Induced Pluripotent Stem Cells Efficiently Differentiate into Neurons Independent of the Number of Reprogramming Factors

Srimasorn,1 Holm Zaehres,5 Peter Reinhardt,5 Hans R. Schöler,5 and Alexander Storch1,2,3,6 1Division for Neurodegenerative Diseases, Department of Neurology, Technische Universität Dresden, Dresden, Germany ... the paper. Hans R. Schöler contributed to provision of study material, critical revision of the paper, and fund raising. Alexander Storch contributed to conception and design, data analysis and

Enhanced OCT4 transcriptional activity substitutes for exogenous SOX2 in cellular reprogramming

Molecular Biomedicine, Röntgenstraße 20, 48149 Münster, GermanyAdele G. Marthaler, Kenjiro Adachi, Guangming Wu, Davood Sabour, Sergiy Velychko, Hans R. Schöler & Natalia TapiaMedical Faculty, Heinrich Heine ... journals • PubMed • Google ScholarSearch for Hans R. Schöler in:Nature Research journals • PubMed • Google ScholarSearch for Natalia Tapia in:Nature Research journals • PubMed • Google Scholar Contributions

Reactivation of the inactive X chromosome and post-transcriptional reprogramming of Xist in iPSCs

Direct reprogramming of somatic cells to pluripotent stem cells entails the obliteration of somatic cell memory and the reestablishment of epigenetic events. Induced pluripotent stem cells (iPSCs) have been created by reprogramming somatic cells through the transduction of reprogramming factors. During cell reprogramming, female somatic cells must overcome at least one more...

Dissecting the role of distinct OCT4-SOX2 heterodimer configurations in pluripotency

The transcription factors OCT4 and SOX2 are required for generating induced pluripotent stem cells (iPSCs) and for maintaining embryonic stem cells (ESCs). OCT4 and SOX2 associate and bind to DNA in different configurations depending on the arrangement of their individual DNA binding elements. Here we have investigated the role of the different OCT4-SOX2-DNA assemblies in...

Generation of integration-free induced hepatocyte-like cells from mouse fibroblasts

The ability to generate integration-free induced hepatocyte-like cells (iHeps) from somatic fibroblasts has the potential to advance their clinical application. Here, we have generated integration-free, functional, and expandable iHeps from mouse somatic fibroblasts. To elicit this direct conversion, we took advantage of an oriP/EBNA1-based episomal system to deliver a set of...

Small Molecule-Assisted, Line-Independent Maintenance of Human Pluripotent Stem Cells in Defined Conditions

Human pluripotent stem cells (hPSCs) are conventionally grown in a mouse feeder cell-dependent manner. Chemically defined culture conditions are, however, desirable not only for potential medically oriented applications but also for investigating mechanisms of self-renewal and differentiation. In light of the rather high complexity and cost of existing defined hPSC culture...

Comprehensive Human Transcription Factor Binding Site Map for Combinatory Binding Motifs Discovery

To know the map between transcription factors (TFs) and their binding sites is essential to reverse engineer the regulation process. Only about 10%–20% of the transcription factor binding motifs (TFBMs) have been reported. This lack of data hinders understanding gene regulation. To address this drawback, we propose a computational method that exploits never used TF properties to...

Establishment of a primed pluripotent epiblast stem cell in FGF4-based conditions

Sung Jung in:Nature Research journals • PubMed • Google ScholarSearch for Jeong Tae Do in:Nature Research journals • PubMed • Google ScholarSearch for Hans R. Schöler in:Nature Research journals • PubMed ... Do or Hans R. Schöler. Supplementary information Word documents1.Supplementary Information Dataset 1 Rights and permissions This work is licensed under a Creative Commons Attribution 4.0

The POU-er of gene nomenclature

The pluripotency factor POU5F1 (OCT4) is well known as a key regulator of stem cell fate. Homologues of POU5F1 exist throughout vertebrates, but the evolutionary and functional relationships between the various family members have been unclear. The level to which function has been conserved within this family provides insight into the evolution of early embryonic potency. Here...

Reprogramming to Pluripotency through a Somatic Stem Cell Intermediate

Transcription factor-based reprogramming can lead to the successful switching of cell fates. We have recently reported that mouse embryonic fibroblasts (MEFs) can be directly reprogrammed into induced neural stem cells (iNSCs) after the forced expression of Brn4, Sox2, Klf4, and Myc. Here, we tested whether iNSCs could be further reprogrammed into induced pluripotent stem cells...

Sox2 Level Is a Determinant of Cellular Reprogramming Potential

Epiblast stem cells (EpiSCs) and embryonic stem cells (ESCs) differ in their in vivo differentiation potential. While ESCs form teratomas and efficiently contribute to the development of chimeras, EpiSCs form teratomas but very rarely chimeras. In contrast to their differentiation potential, the reprogramming potential of EpiSCs has not yet been investigated. Here we demonstrate...