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24 papers found.
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Cryo-EM structure of L-fucokinase/GDP-fucose pyrophosphorylase (FKP) in Bacteroides fragilis

. Wang and J.-W. Wu supervised the experiments and analyzed the data; Y. Liu and L. Sun wrote the paper. Ying Liu, Huifang Hu, Jia Wang, Qiang Zhou, Peng Wu, Nieng Yan, Hong-Wei Wang, Jia-Wei Wu, Linfeng

Real-time shear wave elastography (SWE) assessment of short- and long-term treatment outcome in Budd-Chiari syndrome: A pilot study

Hospital, Beijing, China) for his help with experimental design. Author Contributions Conceptualization: Jie Tang. Data curation: Hong-Wei Wang. Formal analysis: Jia Cheng. Investigation: Hong-Wei Wang ... , Hua-Ning Shi. Methodology: Hong-Wei Wang, Yu-Kun Luo. Resources: Hong-Wei Wang, Hua-Ning Shi. Software: Hong-Wei Wang. 9 / 11 Supervision: Fang Xie. Validation: Fang Xie. Writing ? original draft

Particle segmentation algorithm for flexible single particle reconstruction

As single particle cryo-electron microscopy has evolved to a new era of atomic resolution, sample heterogeneity still imposes a major limit to the resolution of many macromolecular complexes, especially those with continuous conformational flexibility. Here, we describe a particle segmentation algorithm towards solving structures of molecules composed of several parts that are...

Cryo-EM structure and biochemical analysis reveal the basis of the functional difference between human PI3KC3-C1 and -C2

Phosphatidylinositol 3-phosphate (PI3P) plays essential roles in vesicular trafficking, organelle biogenesis and autophagy. Two class III phosphatidylinositol 3-kinase (PI3KC3) complexes have been identified in mammals, the ATG14L complex (PI3KC3-C1) and the UVRAG complex (PI3KC3-C2). PI3KC3-C1 is crucial for autophagosome biogenesis, and PI3KC3-C2 is involved in various membrane...

Complex structure of the fission yeast SREBP-SCAP binding domains reveals an oligomeric organization

Sterol regulatory element-binding protein (SREBP) transcription factors are master regulators of cellular lipid homeostasis in mammals and oxygen-responsive regulators of hypoxic adaptation in fungi. SREBP C-terminus binds to the WD40 domain of SREBP cleavage-activating protein (SCAP), which confers sterol regulation by controlling the ER-to-Golgi transport of the SREBP-SCAP...

Structure of precursor microRNA’s terminal loop regulates human Dicer’s dicing activity by switching DExH/D domain

and Hong-Wei Wang declare that they have no conflict of interest. This article does not contain any studies with human or animal subjects performed by the any of the authors. This article is

4.4 Å Resolution Cryo-EM structure of human mTOR Complex 1

Jiawei Wang 5 Yanhui Xu 0 1 2 Hong-Wei Wang 5 0 State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Fudan University ... financial interests. COMPLIANCE WITH ETHICS GUIDELINES Huirong Yang, Jia Wang, Mengjie Liu, Xizi Chen, Min Huang, Dan Tan, Meng-Qiu Dong, Catherine C. L. Wong, Jiawei Wang, Yanhui Xu and Hong-Wei Wang

Evaluation of Liver Ischemia-Reperfusion Injury in Rabbits Using a Nanoscale Ultrasound Contrast Agent Targeting ICAM-1

Objective To assess the feasibility of ultrasound molecular imaging in the early diagnosis of liver ischemia-reperfusion injury (IRI) using a nanoscale contrast agent targeting anti-intracellular adhesion molecule-1 (anti-ICAM-1). Methods The targeted nanobubbles containing anti-ICAM-1 antibody were prepared using the avidin-biotin binding method. Human hepatic sinusoidal...

Cryo-EM structure of SNAP-SNARE assembly in 20S particle

N-ethylmaleimide-sensitive factor (NSF) and α soluble NSF attachment proteins (α-SNAPs) work together within a 20S particle to disassemble and recycle the SNAP receptor (SNARE) complex after intracellular membrane fusion. To understand the disassembly mechanism of the SNARE complex by NSF and α-SNAP, we performed single-particle cryo-electron microscopy analysis of 20S particles...

Effects of Miglitol, Acarbose, and Sitagliptin on Plasma Insulin and Gut Peptides in Type 2 Diabetes Mellitus: A Crossover Study

. Hiroaki Ueno, Wakaba Tsuchimochi, Hong-Wei Wang, Eiichiro Yamashita, Chikako Tsubouchi, Kazuhiro Nagamine, Hideyuki Sakoda, and Masamitsu Nakazato declare no conflicts of interest. Compliance with ethics

Nuclear factor-κB activation in perihematomal brain tissue correlates with outcome in patients with intracerebral hemorrhage

Background Nuclear factor-κB (NF-κB) plays an important role in the inflammatory response after intracerebral hemorrhage (ICH). We therefore proposed that NF-κB activation in perihematomal brain tissue might correlate with clinical outcome in patients with ICH. To confirm this, we studied clinical data of 45 patients with ICH and NF-κB activation in perihematomal brain tissue and...

The M235T polymorphism in the angiotensinogen gene and heart failure: a meta-analysis

textbooks, review articles and included articles were identified through manual searches to find other potentially eligible studies. Data extraction Two investigators (Song Chen and Hong-Wei Wang) extracted

CryoEM structure of yeast cytoplasmic exosome complex

The eukaryotic multi-subunit RNA exosome complex plays crucial roles in 3′-to-5′ RNA processing and decay. Rrp6 and Ski7 are the major cofactors for the nuclear and cytoplasmic exosomes, respectively. In the cytoplasm, Ski7 helps the exosome to target mRNAs for degradation and turnover via a through-core pathway. However, the interaction between Ski7 and the exosome complex has...

New triterpenoids from the kernels of Azadirachta indica

Three new limonoids (1–3) and a new intact triterpenoid (4), along with three known constituents (5–7), were isolated from the dried kernels (after extracting azadirachtin) of Azadirachta indica. The structures of the new compounds 1-benzoyl-3-deacetyl-1-detigloyl salannin (1), 7-tigloyl-12-oxo vilasini (2), azadiralactone (3) and azadirahemiacetal (4) were elucidated by means of...

Simulations of Tubulin Sheet Polymers as Possible Structural Intermediates in Microtubule Assembly

The microtubule assembly process has been extensively studied, but the underlying molecular mechanism remains poorly understood. The structure of an artificially generated sheet polymer that alternates two types of lateral contacts and that directly converts into microtubules, has been proposed to correspond to the intermediate sheet structure observed during microtubule assembly...

MicroRNA-377 inhibited proliferation and invasion of human glioblastoma cells by directly targeting specificity protein 1

Background Increasing evidence has indicated that microRNAs (miRNAs) are strongly implicated in the initiation and progression of glioblastoma multiforme (GBM). Here, we identified a novel tumor suppressive miRNA, miR-377, and investigated its role and therapeutic effect for GBM.

Mechanistic insights into the role of Hop2–Mnd1 in meiotic homologous DNA pairing

The Hop2–Mnd1 complex functions with the DMC1 recombinase in meiotic recombination. Hop2–Mnd1 stabilizes the DMC1-single-stranded DNA (ssDNA) filament and promotes the capture of the double-stranded DNA partner by the recombinase filament to assemble the synaptic complex. Herein, we define the action mechanism of Hop2–Mnd1 in DMC1-mediated recombination. Small angle X-ray...

Structural insight of a concentration-dependent mechanism by which YdiV inhibits Escherichia coli flagellum biogenesis and motility

YdiV is a negative regulator of cell motility. It interacts with FlhD4C2 complex, a product of flagellar master operon, which works as the transcription activator of all other flagellar operons. Here, we report the crystal structures of YdiV and YdiV2–FlhD2 complex at 1.9 Å and 2.9 Å resolutions, respectively. Interestingly, YdiV formed multiple types of complexes with FlhD4C2...