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Conserved microstructure of the Brassica B Genome of Brassica nigra in relation to homologous regions of Arabidopsis thaliana, B. rapa and B. oleracea

Background The Brassica B genome is known to carry several important traits, yet there has been limited analyses of its underlying genome structure, especially in comparison to the closely related A and C genomes. A bacterial artificial chromosome (BAC) library of Brassica nigra was developed and screened with 17 genes from a 222 kb region of A. thaliana that had been well...

Differential SAGE analysis in Arabidopsis uncovers increased transcriptome complexity in response to low temperature

Background Abiotic stress, including low temperature, limits the productivity and geographical distribution of plants, which has led to significant interest in understanding the complex processes that allow plants to adapt to such stresses. The wide range of physiological, biochemical and molecular changes that occur in plants exposed to low temperature require a robust global...

Arabidopsis mutant sk156 reveals complex regulation of SPL15 in a miR156-controlled gene network

Background The Arabidopsis microRNA156 (miR156) regulates 11 members of the SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) family by base pairing to complementary target mRNAs. Each SPL gene further regulates a set of other genes; thus, miR156 controls numerous genes through a complex gene regulation network. Increased axillary branching occurs in transgenic Arabidopsis...

Integration of linkage maps for the Amphidiploid Brassica napus and comparative mapping with Arabidopsis and Brassica rapa

Background The large number of genetic linkage maps representing Brassica chromosomes constitute a potential platform for studying crop traits and genome evolution within Brassicaceae. However, the alignment of existing maps remains a major challenge. The integration of these genetic maps will enhance genetic resolution, and provide a means to navigate between sequence-tagged...

A consensus map of rapeseed (Brassica napus L.) based on diversity array technology markers: applications in genetic dissection of qualitative and quantitative traits

Harsh Raman 0 Rosy Raman 0 Andrzej Kilian Frank Detering Yan Long David Edwards Isobel AP Parkin Andrew G Sharpe Matthew N Nelson Nick Larkan Jun Zou Jinling Meng M Naveed Aslam Jacqueline Batley

Transcriptome and methylome profiling reveals relics of genome dominance in the mesopolyploid Brassica oleracea

Background Brassica oleracea is a valuable vegetable species that has contributed to human health and nutrition for hundreds of years and comprises multiple distinct cultivar groups with diverse morphological and phytochemical attributes. In addition to this phenotypic wealth, B. oleracea offers unique insights into polyploid evolution, as it results from multiple ancestral...

An archived activation tagged population of Arabidopsis thaliana to facilitate forward genetics approaches

Background Functional genomics tools provide researchers with the ability to apply high-throughput techniques to determine the function and interaction of a diverse range of genes. Mutagenised plant populations are one such resource that facilitate gene characterisation. They allow complex physiological responses to be correlated with the expression of single genes in planta...

Sequence and structure of Brassica rapa chromosome A3

Yang Hee-Ju Yu Ik-Young Choi Beom-Soon Choi Su Ryun Choi Nirala Ramchiary Yong Pyo Lim Fiona Fraser Nizar Drou Eleni Soumpourou Martin Trick Ian Bancroft Andrew G Sharpe Isobel AP Parkin Jacqueline