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Prognostic Impact of the Findings on Thin-Section Computed Tomography in stage I lung adenocarcinoma with visceral pleural invasion

Guarantors of integrity of entire study:Mei Yuan, Jin-Yuan Liu, Teng Zhang, Tong-Fu Yu; study concepts/study design or data acquisition or data analysis/interpretation: all authors; manuscript drafting or ... resolved: all authors; literature research: Mei Yuan, Jin-Yuan Liu, Teng Zhang, Tong-Fu Yu; clinical studies: Mei Yuan, Jin-Yuan Liu, Teng Zhang, Yu-Dong Zhang, Tong-Fu Yu; statistical analysis: Mei Yuan

Comparative Genome-Wide Analysis of the Malate Dehydrogenase Gene Families in Cotton

Malate dehydrogenases (MDHs) play crucial roles in the physiological processes of plant growth and development. In this study, 13 and 25 MDH genes were identified from Gossypium raimondii and Gossypium hirsutum, respectively. Using these and 13 previously reported Gossypium arboretum MDH genes, a comparative molecular analysis between identified MDH genes from G. raimondii, G...

Genome-Wide Comparative Analysis of the Phospholipase D Gene Families among Allotetraploid Cotton and Its Diploid Progenitors

-Juan Dong Jin-Yuan Liu 0 0 Laboratory of Plant Molecular Biology, Center for Plant Biology, School of Life Sciences, Tsinghua University , Beijing , China , 2 Institute of Vegetables and Flowers

Comparative proteomic analyses of Asian cotton ovules with attached fibers in the early stages of fiber elongation process

Jin-Yuan Liu 0 0 Laboratory of Plant Molecular Biology, Center for Plant Biology, School of Life Sciences, Tsinghua University , Beijing 100084 , People's Republic of China Background: Plenty of

Comparative proteomic analyses of Asian cotton ovules with attached fibers in the early stages of fiber elongation process

Plenty of proteomic studies were performed to characterize the allotetraploid upland cotton fiber elongation process, whereas little is known about the elongating diploid cotton fiber proteome. In this study, we used a two-dimensional electrophoresis-based comparative proteomic approach to profile dynamic proteomes of diploid Asian cotton ovules with attached fibers in the early...

GhMCS1, the Cotton Orthologue of Human GRIM-19, Is a Subunit of Mitochondrial Complex I and Associated with Cotton Fibre Growth

GRIM-19 (Gene associated with Retinoid-Interferon-induced Mortality 19) is a subunit of mitochondrial respiratory complex I in mammalian systems, and it has been demonstrated to be a multifunctional protein involved in the cell cycle, cell motility and innate immunity. However, little is known about the molecular functions of its homologues in plants. Here, we characterised...

Mass Spectrometric Identification of In Vivo Phosphorylation Sites of Differentially Expressed Proteins in Elongating Cotton Fiber Cells

Two-dimensional gel electrophoresis (2-DE)-based proteomics approach was applied to extensively explore the molecular basis of plant development and environmental adaptation. These proteomics analyses revealed thousands of differentially expressed proteins (DEPs) closely related to different biological processes. However, little attention has been paid to how peptide mass...

Comparative Proteomic and Biochemical Analyses Reveal Different Molecular Events Occurring in the Process of Fiber Initiation between Wild-Type Allotetraploid Cotton and Its Fuzzless-Lintless Mutant

To explore lint fiber initiation-related proteins in allotetraploid cotton (Gossypium hirsutum L.), a comparative proteomic analysis was performed between wild-type cotton (Xu-142) and its fuzzless-lintless mutant (Xu-142-fl) at five developmental time points for lint fiber initiation from -3 to +3 days post-anthesis (dpa). Using two-dimensional gel electrophoresis (2-DE...

Genome-wide analysis of small RNAs reveals eight fiber elongation-related and 257 novel microRNAs in elongating cotton fiber cells

Wei Xue 0 Zhengming Wang 0 Mingjian Du Yidi Liu Jin-Yuan Liu 0 Equal contributors Laboratory of Molecular Biology, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University

Core-Shell Structure of Gold Nanoparticles with Inositol Hexaphosphate Nanohybrids for Label-Free and Rapid Detection by SERS Nanotechnology

Gold nanoparticles bound with inositol hexaphosphate (IP6) (AuNPs/IP6) were prepared by in situ reduction of various concentrations of IP6 (0~320 µM) through modified Frens method for surface-enhanced Raman scattering (SERS) detection. The resultant AuNPs/IP6 were subject to characterization including UV/Vis spectroscopy, transmission electron microscopy (TEM), dynamic light...

Core-Shell Structure of Gold Nanoparticles with Inositol Hexaphosphate Nanohybrids for Label-Free and Rapid Detection by SERS Nanotechnology

Gold nanoparticles bound with inositol hexaphosphate (IP6) (AuNPs/IP6) were prepared by in situ reduction of various concentrations of IP6 (0~320 µM) through modified Frens method for surface-enhanced Raman scattering (SERS) detection. The resultant AuNPs/IP6 were subject to characterization including UV/Vis spectroscopy, transmission electron microscopy (TEM), dynamic light...

Core-Shell Structure of Gold Nanoparticles with Inositol Hexaphosphate Nanohybrids for Label-Free and Rapid Detection by SERS Nanotechnology

Gold nanoparticles bound with inositol hexaphosphate (IP6) (AuNPs/IP6) were prepared by in situ reduction of various concentrations of IP6 (0~320 µM) through modified Frens method for surface-enhanced Raman scattering (SERS) detection. The resultant AuNPs/IP6 were subject to characterization including UV/Vis spectroscopy, transmission electron microscopy (TEM), dynamic light...

The Arabidopsis EAR-motif-containing protein RAP2.1 functions as an active transcriptional repressor to keep stress responses under tight control

Background Plants respond to abiotic stress through complex regulation of transcription, including both transcriptional activation and repression. Dehydration-responsive-element binding protein (DREB)-type transcription factors are well known to play important roles in adaptation to abiotic stress. The mechanisms by which DREB-type transcription factors activate stress-induced...

Identification and analysis of seven H2O2-responsive miRNAs and 32 new miRNAs in the seedlings of rice (Oryza sativa L. ssp. indica)

Plant microRNAs (miRNAs) have been shown to play critical roles in regulating gene expression at the post-transcriptional level. In this study, we employed high throughput sequencing combined with computational analysis to survey miRNAomes from the seedlings of rice under normal conditions and treatments of H2O2 that result in oxidative stress. Comparison of the miRNAomes and...

Comparative Proteomics Analysis of the Root Apoplasts of Rice Seedlings in Response to Hydrogen Peroxide

Background Plant apoplast is the prime site for signal perception and defense response, and of great importance in responding to environmental stresses. Hydrogen peroxide (H2O2) plays a pivotal role in determining the responsiveness of cells to stress. However, how the apoplast proteome changes under oxidative condition is largely unknown. In this study, we initiated a...

A Comparative miRNAome Analysis Reveals Seven Fiber Initiation-Related and 36 Novel miRNAs in Developing Cotton Ovules

An increasing number of microRNAs (miRNAs) have been shown to play crucial regulatory roles in the process of plant development. Here, we used high-throughput sequencing combined with computational analysis to characterize miRNAomes from the ovules of wild-type upland cotton and a fiberless mutant during fiber initiation. Comparative miRNAome analysis combined with northern...