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Exploring PEGylated and immobilized laccases for catechol polymerization

Laccases have been reported for their ability to eliminate hazardous phenolic compounds by oxidative polymerization. The exploitation of the oxidative behavior of different laccase forms, namely free/native, free/PEGylated, immobilized/native and immobilized/PEGylated, was assessed in this study. We found that PEGylated and immobilized laccase forms have differentiated catalytic...

Ultrasound-assisted extraction of hemicellulose and phenolic compounds from bamboo bast fiber powder

Tallian, Jing Su, Artur Cavaco-Paulo. Visualization: Jiajia Fu. Writing ? original draft: Claudia Tallian. 9 / 11 Writing ? review & editing: Claudia Tallian, Jing Su, Robert Vielnascher, Carla Silva

Bio-coloration of bacterial cellulose assisted by immobilized laccase

In this work a process for the bio-coloration of bacterial cellulose (BC) membranes was developed. Laccase from Myceliophthora thermophila was immobilized onto BC membranes and retained up to 88% of residual activity after immobilization. Four compounds belonging to the flavonoids family were chosen to test the in situ polymerase activity of immobilized laccase. All the...

Attenuated expression of MTR in both prenatally androgenized mice and women with the hyperandrogenic phenotype of PCOS

, Jianjun Zhou. Funding acquisition: Haixiang Sun. Investigation: Lei Lei. Methodology: Lei Lei, Lijun Ding, Jing Su. Project administration: Haixiang Sun, Guijun Yan. Resources: Qingqing Shi, Jianjun

A Graphene Oxide-Based Fluorescent Aptasensor for the Turn-on Detection of CCRF-CEM

A convenient, low-cost, and highly sensitive fluorescent aptasensor for detection of leukemia has been developed based on graphene oxide-aptamer complex (GO-apt). Graphene oxide (GO) can absorb carboxyfluorescein-labeled Sgc8 aptamer (FAM-apt) by π-π stacking and quench the fluorescence through fluorescence resonance energy transfer (FRET). In the absence of Sgc8 target cell CCRF...

Dicumarol inhibits PDK1 and targets multiple malignant behaviors of ovarian cancer cells

Pyruvate dehydrogenase kinase 1 (PDK1) is overexpressed in ovarian cancer and thus is a promising anticancer therapeutic target. Our previous work suggests that coumarin compounds are potential inhibitors of PDKs. In this study, we used the ovarian cancer cell line SKOV3 as the model system and examined whether dicumarol (DIC), a coumarin compound, could inhibit ovarian cancer...

A systems biology approach to studying the molecular mechanisms of osteoblastic differentiation under cytokine combination treatment

Recent studies revealed that sequential release of bone morphogenetic protein 2 and insulin-like growth factor 1 plays an important role in osteogenic process, suggesting that cytokines bone morphogenetic protein 2 and insulin-like growth factor 1 function in a time-dependent manner. However, the specific molecular mechanisms underlying these observations remained elusive...

C1QBP suppresses cell adhesion and metastasis of renal carcinoma cells

); Yong Wang, Donghe Fu, Jing Su, Yajing Chen, Can Qi, Yin Sun and Yuanjie Niu (acquisition of data, analysis and interpretation of data, statistical analysis, drafting of the manuscript); All authors

Autophagy inhibitor chloroquine increases sensitivity to cisplatin in QBC939 cholangiocarcinoma cells by mitochondrial ROS

The tumor cells have some metabolic characteristics of the original tissues, and the metabolism of the tumor cells is closely related to autophagy. However, the mechanism of autophagy and metabolism in chemotherapeutic drug resistance is still poorly understood. In this study, we investigated the role and mechanism of autophagy and glucose metabolism in chemotherapeutic drug...

Fusions of Tumor-derived Endothelial Cells with Dendritic Cells Induces Antitumor Immunity

To explore dendritic cells/tumor-derived endothelial cells (DC/EC) fusion cells are potent stimulators of T cells to impact tumor progression. ECs were isolated from mice hepatoma cell line (H22) Xenograft, and dendritic cells were isolated from bone marrow of BALB/c mice, then the isolated ECs were cultured and detected the endothelial surface expression of CD105 by flow...

Relational Network for Knowledge Discovery through Heterogeneous Biomedical and Clinical Features

Biomedical big data, as a whole, covers numerous features, while each dataset specifically delineates part of them. “Full feature spectrum” knowledge discovery across heterogeneous data sources remains a major challenge. We developed a method called bootstrapping for unified feature association measurement (BUFAM) for pairwise association analysis, and relational dependency...

The Application of Cloud Innovation in Industrial Design Education

According to the domestic status of students’ study and practice of industrial design, the application of cloud innovation in industrial design education was proposed to realize online design reviews, dissemination and sharing, virtual display and demand-oriented collaborative design. The basic framework and function of cloud innovation system were analyzed to solve three...

The Application of Cloud Innovation in Industrial Design Education

According to the domestic status of students’ study and practice of industrial design, the application of cloud innovation in industrial design education was proposed to realize online design reviews, dissemination and sharing, virtual display and demand-oriented collaborative design. The basic framework and function of cloud innovation system were analyzed to solve three...

Vitrectomy with internal limiting membrane peeling vs no peeling for Macular Hole-induced Retinal Detachment (MHRD): a meta-analysis

Background we conducted our meta-analysis of published studies to assess existing evidence about the efficacy and safety of vitrectomy with ILM peeling vs. that of vitrectomy with no ILM peeling for Macular hole-induced retinal detachment. Methods Databases, including Pubmed, Cochrane Library, Ovid, Web of Science, Wanfang and CNKI, were searched to identify studies comparing...

Transmembrane protein 106a activates mouse peritoneal macrophages via the MAPK and NF-κB signaling pathways

The M1 and M2 states of macrophage are the two extremes of a physiologic/phenotypic continuum that is dynamically influenced by environmental signals. Molecular mechanism analysis indicated that they gain M1 and M2-related functions after encountering specific ligands in the tissue environment. Here, we first characterized the previously unknown immunobiological functions of...

Decreased miR-204 in H. pylori-Associated Gastric Cancer Promotes Cancer Cell Proliferation and Invasion by Targeting SOX4

Background The molecular mechanism between Helicobacter pylori (H. pylori) infection and gastric cancer remained largely unknown. In this study, we determined the role of miRNA in H. pylori induced gastric cancer. Methods and Results We found that miR-204 was decreased in H. pylori positive tissues by qRT-PCR. Knockdown of miR-204 enhanced the invasion and proliferation ability...

Intravenous Remifentanil versus Epidural Ropivacaine with Sufentanil for Labour Analgesia: A Retrospective Study

Remifentanil with appropriate pharmacological properties seems to be an ideal alternative to epidural analgesia during labour. A retrospective cohort study was undertaken to assess the efficacy and safety of remifentanil intravenous patient-controlled analgesia (IVPCA) compared with epidural analgesia. Medical records of 370 primiparas who received remifentanil IVPCA or epidural...

Antibacterial small molecules targeting the conserved TOPRIM domain of DNA gyrase

administration: Charles G. Garlisi, Jing Su, Christopher M. Tan, Todd A. Black, Terry Resources: Nicholas Murgolo, Charles G. Garlisi, Jing Su, Stacia Kargman, Payal Sheth, Nichole Buist, Todd A. Black