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Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultry

Chitin, a polymer of N-acetyl-D-glucosamine (GlcNAc), functions as a major structural component in crustaceans, insects and fungi and is the second most abundant polysaccharide in the nature. Although these chitin-containing organisms have been suggested as novel animal feed resources, chitin has long been considered as indigestible fibers in the animal body. Recently, we...

Protease resistance of porcine acidic mammalian chitinase under gastrointestinal conditions implies that chitin-containing organisms can be sustainable dietary resources

Life Science, Kogakuin University, Hachioji, Tokyo, 192-0015, JapanEri Tabata, Akinori Kashimura, Satoshi Wakita, Misa Ohno, Masayoshi Sakaguchi, Yasusato Sugahara, Yasutada Imamura & Fumitaka ... • PubMed • Google Scholar Search for Masayoshi Sakaguchi in:Nature Research journals • PubMed • Google Scholar Search for Yasusato Sugahara in:Nature Research journals • PubMed • Google Scholar Search for

Characterization of a Bacillus thuringiensis chitinase that binds to cellulose and chitin

Masao Kawakita Fumitaka Oyama 0 Masayoshi Sakaguchi 0 0 Department of Chemistry and Life Science, Kogakuin University , 2, 665-1 Nakano-cho, Hachioji, Tokyo 192-0015 , Japan Bacillus thuringiensis is a

Loss and Gain of Human Acidic Mammalian Chitinase Activity by Nonsynonymous SNPs

Acidic mammalian chitinase (AMCase) is implicated in asthma, allergic inflammation, and food processing. Little is known about genetic and evolutional regulation of chitinolytic activity of AMCase. Here, we relate human AMCase polymorphisms to the mouse AMCase, and show that the highly active variants encoded by nonsynonymous single-nucleotide polymorphisms (nsSNPs) are...

Establishment of a quantitative PCR system for discriminating chitinase-like proteins: catalytically inactive breast regression protein-39 and Ym1 are constitutive genes in mouse lung

Background Mice and humans produce chitinase-like proteins (CLPs), which are highly homologous to chitinases but lack chitinolytic activity. Mice express primarily three CLPs, including breast regression protein-39 (BRP-39) [chitinase 3-like-1 (Chi3l1) or 38-kDa glycoprotein (gp38k)], Ym1 (Chi3l3) and Ym2 (Chi3l4). Recently, CLPs have attracted considerable attention due to their...

Acidic mammalian chitinase is a proteases-resistant glycosidase in mouse digestive system

, Riho Onuki, Chiyuki Nemoto, Eri Tabata, Satoshi Wakita, Akinori Kashimura, Masayoshi Sakaguchi, Yasusato Sugahara & Fumitaka OyamaLaboratory for Structural Neuropathology, RIKEN Brain Science Institute ... ScholarSearch for Satoshi Wakita in:Nature Research journals • PubMed • Google ScholarSearch for Akinori Kashimura in:Nature Research journals • PubMed • Google ScholarSearch for Masayoshi Sakaguchi in:Nature

Functional Properties of Mouse Chitotriosidase Expressed in the Periplasmic Space of Escherichia coli

Chitotriosidase (Chit1) is an enzyme associated with various diseases, including Gaucher disease, chronic obstructive pulmonary disease, Alzheimer disease and cystic fibrosis. In this study, we first expressed mouse mature Chit1 fused with V5 and (His)6 tags at the C-terminus (Chit1-V5-His) in the cytoplasm of Escherichia coli and found that most of the expressed protein was...

Highly conserved salt bridge stabilizes a proteinase K subfamily enzyme, Aqualysin I, from Thermus aquaticus YT-1

The proteinase K subfamily enzymes, thermophilic Aqualysin I (AQN) from Thermus aquaticus YT-1 and psychrophilic serine protease (VPR) from Vibrio sp. PA-44, have six and seven salt bridges, respectively. To understand the possible significance of salt bridges in the thermal stability of AQN, we prepared mutant proteins in which amino acid residues participating in salt bridges...

Glucoamylase of Caulobacter crescentus CB15: cloning and expression in Escherichia coli and functional identification

The biochemical properties of the maltodextrin-hydrolyzing enzymes of cold-tolerant proteobacterium Caulobacter crescentus CB15 remain to be elucidated, although whose maltodextrin transport systems were well investigated. We cloned the putative glucoamylase of C. crescentus CB15 (CauloGA) gene. The CauloGA gene product that was expressed in E. coli was prone to forming inclusion...

Chitinase mRNA Levels by Quantitative PCR Using the Single Standard DNA: Acidic Mammalian Chitinase Is a Major Transcript in the Mouse Stomach

Sakaguchi 0 Yasusato Sugahara 0 Fumitaka Oyama 0 Dominik Hartl, University of Tu bingen, Germany 0 Department of Applied Chemistry, Kogakuin University , Hachioji, Tokyo , Japan Chitinases hydrolyze the b-1

Quantification of Chitinase mRNA Levels in Human and Mouse Tissues by Real-Time PCR: Species-Specific Expression of Acidic Mammalian Chitinase in Stomach Tissues

Chitinase hydrolyzes chitin, which is an N-acetyl-D-glucosamine polymer that is present in a wide range of organisms, including insects, parasites and fungi. Although mammals do not contain any endogenous chitin, humans and mice express two active chitinases, chitotriosidase (Chit1) and acidic mammalian chitinase (AMCase). Because the level of expression of these chitinases is...

Protein A-Mouse Acidic Mammalian Chitinase-V5-His Expressed in Periplasmic Space of Escherichia coli Possesses Chitinase Functions Comparable to CHO-Expressed Protein

Acidic mammalian chitinase (AMCase) has been shown to be associated with asthma in mouse models, allergic inflammation and food processing. Here, we describe an E. coli-expression system that allows for the periplasmic production of active AMCase fused to Protein A at the N-terminus and V5 epitope and (His)6 tag (V5-His) at the C-terminus (Protein A-AMCase-V5-His) in E. coli. The...

Amino acid residues important for CMP-sialic acid recognition by the CMP-sialic acid transporter: analysis of the substrate specificity of UDP-galactose/CMP-sialic acid transporter chimeras

In our previous studies, we demonstrated that chimeric molecules of the CMP-sialic acid (CMP-Sia) transporter (CST) and the UDP-galactose (Gal) transporter (UGT) in which the seventh transmembrane helix-containing segment was derived from the CST could transport both CMP-Sia and UDP-Gal and that the CST-derived seventh transmembrane helix segment was sufficient for the chimera to...

Successful Renal Replacement Therapy for a Patient with Severe Hemophilia after Surgical Treatment of Intracranial Hemorrhage and Hydrocephalus

Yasuda,1 Yukiyo Yokomaku,1 Masayoshi Sakaguchi,1 Keiji Isshiki,1 Shin-ichi Araki,1 Shigeru Ohta,2 and Takashi Uzu1 1Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu

A case of post-allogeneic haematopoietic stem cell transplantation membranous nephropathy

, Philadelphia, USA: 1994; 928-1001 3 Department of Nephrology Toshiro Sugimoto Yuki Tanaka Masayoshi Sakaguchi Norihisa Osawa Yuki Tanaka Takashi Uzu Atsunori Kashiwagi - the precipitation (saturation point

Benidipine Attenuates Glomerular Hypertension and Reduces Albuminuria in Patients with Metabolic Syndrome

Recent studies have shown that metabolic syndrome is associated with an increased risk for chronic kidney disease. We recently found that the prevalence of sodium-sensitive hypertension in patients with metabolic syndrome was significantly higher than that in patients with essential hypertension but without metabolic syndrome. We therefore assessed the effects of benidipine, a...