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added); see Michael Seringhaus, Kindle: How To Buy a Book but Not Own It, HARTFORD COURANT, Aug. 5, 2009, at A13, available at A. Applying the U.C.C. As previously stated, the U.C.C. generally governs

Mismatch oligonucleotides in human and yeast: guidelines for probe design on tiling microarrays

Background Mismatched oligonucleotides are widely used on microarrays to differentiate specific from nonspecific hybridization. While many experiments rely on such oligos, the hybridization behavior of various degrees of mismatch (MM) structure has not been extensively studied. Here, we present the results of two large-scale microarray experiments on S. cerevisiae and H. sapiens...

Genomic analysis of insertion behavior and target specificity of mini-Tn7 and Tn3 transposons in Saccharomyces cerevisiae

Transposons are widely employed as tools for gene disruption. Ideally, they should display unbiased insertion behavior, and incorporate readily into any genomic DNA to which they are exposed. However, many transposons preferentially insert at specific nucleotide sequences. It is unclear to what extent such bias affects their usefulness as mutagenesis tools. Here, we examine...