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Search: authors:"Onn Haji Hashim"

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Development of high-performance two-dimensional gel electrophoresis for human hair shaft proteome

, potentially including biomarkers studies related to hair health, diseases and ageing. Author Contributions Conceptualization: Sing Ying Wong, Onn Haji Hashim, Nobuhiro Hayashi. Data curation: Sing Ying Wong ... , Onn Haji Hashim, Nobuhiro Hayashi. Funding acquisition: Nobuhiro Hayashi. Investigation: Sing Ying Wong, Nobuhiro Hayashi. Methodology: Sing Ying Wong, Onn Haji Hashim, Nobuhiro Hayashi. Project

Inverse changes in plasma tetranectin and titin levels in patients with type 2 diabetes mellitus: a potential predictor of acute myocardial infarction?

revised the manuscript from a clinical perspective; Onn Haji HASHIM designed the study, contributed analytic tools, and critically revised the manuscript. All of the authors approved the final manuscript ... Ismail in:Nature Research journals • PubMed • Google Scholar Search for Onn Haji Hashim in:Nature Research journals • PubMed • Google Scholar Corresponding author Correspondence to Onn Haji Hashim

A High-Yield Two-Hour Protocol for Extraction of Human Hair Shaft Proteins

Proteome analysis of the human hair remains challenging due to the poor solubility of hair proteins and the difficulty in their extraction. In the present study, we have developed a rapid extraction protocol for hair shaft protein using alkaline-based buffer. The new protocol accelerated the procedure by reducing the extraction time from at least a day to less than two hours and...

Unmasking Heavily O-Glycosylated Serum Proteins Using Perchloric Acid: Identification of Serum Proteoglycan 4 and Protease C1 Inhibitor as Molecular Indicators for Screening of Breast Cancer

Heavily glycosylated mucin glycopeptides such as CA 27.29 and CA 15–3 are currently being used as biomarkers for detection and monitoring of breast cancer. However, they are not well detected at the early stages of the cancer. In the present study, perchloric acid (PCA) was used to enhance detection of mucin-type O-glycosylated proteins in the serum in an attempt to identify new...

Infection of Burkholderia cepacia Induces Homeostatic Responses in the Host for Their Prolonged Survival: The Microarray Perspective

Burkholderia cepacia is an opportunistic human pathogen associated with life-threatening pulmonary infections in immunocompromised individuals. Pathogenesis of B. cepacia infection involves adherence, colonisation, invasion, survival and persistence in the host. In addition, B. cepacia are also known to secrete factors, which are associated with virulence in the pathogenesis of...

Profiling of Burkholderia cepacia Secretome at Mid-Logarithmic and Early-Stationary Phases of Growth

Background Burkholderia cepacia is a Gram-negative pathogen that causes serious respiratory infections in immunocompromised patients and individuals with cystic fibrosis. This bacterium is known to release extracellular proteins that may be involved in virulence. Methodology/Principal Findings In the present study, B. cepacia grown to mid-logarithmic and early-stationary phases...

Tamarindus indica Extract Alters Release of Alpha Enolase, Apolipoprotein A-I, Transthyretin and Rab GDP Dissociation Inhibitor Beta from HepG2 Cells

Background The plasma cholesterol and triacylglycerol lowering effects of Tamarindus indica extract have been previously described. We have also shown that the methanol extract of T. indica fruit pulp altered the expression of lipid-associated genes including ABCG5 and APOAI in HepG2 cells. In the present study, effects of the same extract on the release of proteins from the...