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Search: authors:"Patricia Pérez"

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Dual control of the levels of photoprotective compounds by ultraviolet radiation and temperature in the freshwater copepod Boeckella antiqua

Photoprotective compounds (PPCs), such as carotenoids and mycosporine-like amino acids (MAAs), confer photoprotection to aquatic organisms against harmful ultraviolet-B (UVB) radiation. The natural variability of these compounds in zooplankton has been related to temperature, radiation and diet, but the ultimate mechanisms regulating the observed patterns in the field are still...

Involvement of phage ϕ29 DNA polymerase and terminal protein subdomains in conferring specificity during initiation of protein-primed DNA replication

To initiate ϕ29 DNA replication, the DNA polymerase has to form a complex with the homologous primer terminal protein (TP) that further recognizes the replication origins of the homologous TP-DNA placed at both ends of the linear genome. By means of chimerical proteins, constructed by swapping the priming domain of the related ϕ29 and GA-1 TPs, we show that DNA polymerase can...

Involvement of φ29 DNA polymerase thumb subdomain in the proper coordination of synthesis and degradation during DNA replication

φ29 DNA polymerase achieves a functional coupling between its 3′–5′ exonuclease and polymerization activities by means of important contacts with the DNA at both active sites. The placement and orientation of residues Lys538, Lys555, Lys557, Gln560, Thr571, Thr573 and Lys575 in a modelled φ29 DNA polymerase–DNA complex suggest a DNA-binding role. In addition, crystal structure of...