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Search: authors:"Rogelio Hernández-Tamayo"

2 papers found.
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Bacillus subtilis RarA forms damage-inducible foci that scan the entire cell

ObjectivesLittle is known about the activity and dynamics of ATPase RarA in B. subtilis, proposed to act at stalled DNA replication forks due to DNA damage. We performed fluorescence microscopy time lapse experiments with a functional RarA-mVenus fusion to visualize the dynamics of RarA during conditions that generate DNA damage.Data descriptionIn exponentially growing cells, we...

Site-specific bacterial chromosome engineering mediated by IntA integrase from Rhizobium etli

Background The bacterial chromosome may be used to stably maintain foreign DNA in the mega-base range. Integration into the chromosome circumvents issues such as plasmid replication, stability, incompatibility, and copy number variance. The site-specific integrase IntA from Rhizobium etli CFN42 catalyzes a direct recombination between two specific DNA sites: attA and attD (23 bp...