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Anguillicola crassus infection affects mRNA expression levels in gas gland tissue of European yellow and silver eel

Fisheries Ecology, Hamburg, Germany, for providing eels. Author Contributions Conceptualization: Bernd Pelster. Data curation: Gabriel Schneebauer, Ron P. Dirks. Formal analysis: Gabriel Schneebauer, Ron P ... . Dirks. Funding acquisition: Bernd Pelster. Investigation: Gabriel Schneebauer. Methodology: Ron P. Dirks. Project administration: Bernd Pelster. Resources: Bernd Pelster. 22 / 26 Supervision: Bernd

The Olfactory Transcriptome and Progression of Sexual Maturation in Homing Chum Salmon Oncorhynchus keta

Reproductive homing migration of salmonids requires accurate interaction between the reception of external olfactory cues for navigation to the spawning grounds and the regulation of sexual maturation processes. This study aimed at providing insights into the hypothesized functional link between olfactory sensing of the spawning ground and final sexual maturation. We have...

Advances in genomics of bony fish

Herman P. Spaink Hans J. Jansen Ron P. Dirks In this review, we present an overview of the recent advances of genomic technologies applied to studies of fish species belonging to the superclass of

Genomics in Eels — Towards Aquaculture and Biology

Yuki Minegishi Christiaan V. Henkel Ron P. Dirks Guido E. E. J. M. van den Thillart Freshwater eels (genus Anguilla), especially the species inhabiting the temperate areas such as the European

Parallel deep transcriptome and proteome analysis of zebrafish larvae

Background Sensitivity and throughput of transcriptomic and proteomic technologies have advanced tremendously in recent years. With the use of deep sequencing of RNA samples (RNA-seq) and mass spectrometry technology for protein identification and quantitation, it is now feasible to compare gene and protein expression on a massive scale and for any organism for which genomic data...

An Atypical Unfolded Protein Response in Heat Shocked Cells

Background The heat shock response (HSR) and the unfolded protein response (UPR) are both activated by proteotoxic stress, although in different compartments, and share cellular resources. How these resources are allocated when both responses are active is not known. Insight in possible crosstalk will help understanding the consequences of failure of these systems in (age-related...

Manipulating Heat Shock Factor-1 in Xenopus Tadpoles: Neuronal Tissues Are Refractory to Exogenous Expression

Background The aging related decline of heat shock factor-1 (HSF1) signaling may be causally related to protein aggregation diseases. To model such disease, we tried to cripple HSF1 signaling in the Xenopus tadpole. Results Over-expression of heat shock factor binding protein-1 did not inhibit the heat shock response in Xenopus. RNAi against HSF1 mRNA inhibited the heat shock...

The Pituitary Gland of the European Eel Reveals Massive Expression of Genes Involved in the Melanocortin System

Hormones secreted from the pituitary gland regulate important processes such as development, growth and metabolism, reproduction, water balance, and body pigmentation. Synthesis and secretion of pituitary hormones are regulated by different factors from the hypothalamus, but also through feedback mechanisms from peripheral organs, and from the pituitary itself. In the European...

Co-chaperones are limiting in a depleted chaperone network

To probe the limiting nodes in the chaperoning network which maintains cellular proteostasis, we expressed a dominant negative mutant of heat shock factor 1 (dnHSF1), the regulator of the cytoplasmic proteotoxic stress response. Microarray analysis of non-stressed dnHSF1 cells showed a two- or more fold decrease in the transcript level of 10 genes, amongst which are the (co...

First artificial hybrid of the eel species Anguilla australis and Anguilla anguilla

Background Studies on artificial hybridization of different Anguilla species were conducted recently, i.e. female A. australis with male A. dieffenbachii, and female A. japonica with male A. anguilla. The existence of these artificial hybrids was however not demonstrated by independent genetic methods. Two species - A. anguilla and A. australis - that are phylogenetically close...

A High-Throughput Screen for Tuberculosis Progression

One-third of the world population is infected with Mycobacterium tuberculosis and multi-drug resistant strains are rapidly evolving. The noticeable absence of a whole organism high-throughput screening system for studying the progression of tuberculosis is fast becoming the bottleneck in tuberculosis research. We successfully developed such a system using the zebrafish...

Primitive Duplicate Hox Clusters in the European Eel's Genome

The enigmatic life cycle and elongated body of the European eel (Anguilla anguilla L., 1758) have long motivated scientific enquiry. Recently, eel research has gained in urgency, as the population has dwindled to the point of critical endangerment. We have assembled a draft genome in order to facilitate advances in all provinces of eel biology. Here, we use the genome to...