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Search: authors:"Sing Ying Wong"

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Development of high-performance two-dimensional gel electrophoresis for human hair shaft proteome

, potentially including biomarkers studies related to hair health, diseases and ageing. Author Contributions Conceptualization: Sing Ying Wong, Onn Haji Hashim, Nobuhiro Hayashi. Data curation: Sing Ying Wong ... , Onn Haji Hashim, Nobuhiro Hayashi. Funding acquisition: Nobuhiro Hayashi. Investigation: Sing Ying Wong, Nobuhiro Hayashi. Methodology: Sing Ying Wong, Onn Haji Hashim, Nobuhiro Hayashi. Project

A High-Yield Two-Hour Protocol for Extraction of Human Hair Shaft Proteins

Proteome analysis of the human hair remains challenging due to the poor solubility of hair proteins and the difficulty in their extraction. In the present study, we have developed a rapid extraction protocol for hair shaft protein using alkaline-based buffer. The new protocol accelerated the procedure by reducing the extraction time from at least a day to less than two hours and...