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Zika convalescent macaques display delayed induction of anamnestic cross-neutralizing antibody responses after dengue infection

Dana L. Vanlandingham in:Nature Research journals • PubMed • Google Scholar Search for Stephen Higgs in:Nature Research journals • PubMed • Google Scholar Search for Mark G. Lewis in:Nature Research

Culex tarsalis is a competent vector species for Cache Valley virus

Cache Valley virus (CVV) is a mosquito-borne orthobunyavirus endemic in North America. The virus is an important agricultural pathogen leading to abortion and embryonic lethality in ruminant species, especially sheep. The importance of CVV in human public health has recently increased because of the report of severe neurotropic diseases. However, mosquito species responsible for...

North American domestic pigs are susceptible to experimental infection with Japanese encephalitis virus

, USASo Lee Park, Yan-Jang S. Huang, Amy C. Lyons, Victoria B. Ayers, D. Scott McVey, Stephen Higgs & Dana L. VanlandinghamBiosecurity Research Institute, Kansas State University, Manhattan, KS, USASo Lee ... Park, Yan-Jang S. Huang, Amy C. Lyons, Victoria B. Ayers, Susan M. Hettenbach, Kenneth R. Burton, Stephen Higgs & Dana L. VanlandinghamArthropod-Borne Animal Diseases Research Unit, Center for Grain and

Differential outcomes of Zika virus infection in Aedes aegypti orally challenged with infectious blood meals and infectious protein meals

, Christopher Clarkston, Brittany Hilfiker, Kathryn Ryan, and Sydney Rathjen for their technical assistance. 7 / 9 Author Contributions Conceptualization: Yan-Jang S. Huang, Stephen Higgs, Dana L ... . Vanlandingham. Formal analysis: Yan-Jang S. Huang, Wei-Wen Hsu. Funding acquisition: Stephen Higgs, Dana L. Vanlandingham. Investigation: Yan-Jang S. Huang, Amy C. Lyons, So Lee Park. Resources: Stephen Higgs

Mutagenesis analysis of T380R mutation in the envelope protein of yellow fever virus

Stephen Higgs 0 1 0 Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University , Manhattan, KS 66506 , USA 1 Biosecurity Research Institute, Kansas State

pH-Dependent entry of chikungunya virus fusion into mosquito cells

Background Millions of human infections caused by arthropod-borne pathogens are initiated by the feeding of an infected mosquito on a vertebrate. However, interactions between the viruses and the mosquito vector, which facilitates successful infection and transmission of virus to a subsequent vertebrate host, are still not fully understood. Finding Here we describe early...

Chikungunya virus adaptation to Aedes albopictus mosquitoes does not correlate with acquisition of cholesterol dependence or decreased pH threshold for fusion reaction

Background Chikungunya virus (CHIKV) is a mosquito transmitted alphavirus that recently caused several large scale outbreaks/epidemics of arthritic disease in tropics of Africa, Indian Ocean basin and South-East Asia. This re-emergence event was facilitated by genetic adaptation (E1-A226V substitution) of CHIKV to a newly significant mosquito vector for this virus; Aedes...

Effective suppression of Dengue virus using a novel group-I intron that induces apoptotic cell death upon infection through conditional expression of the Bax C-terminal domain

Introduction Approximately 100 million confirmed infections and 20,000 deaths are caused by Dengue virus (DENV) outbreaks annually. Global warming and rapid dispersal have resulted in DENV epidemics in formally non-endemic regions. Currently no consistently effective preventive measures for DENV exist, prompting development of transgenic and paratransgenic vector control...

Deliberate Attenuation of Chikungunya Virus by Adaptation to Heparan Sulfate-Dependent Infectivity: A Model for Rational Arboviral Vaccine Design

Mosquito-borne chikungunya virus (CHIKV) is a positive-sense, single-stranded RNA virus from the genus Alphavirus, family Togaviridae, which causes fever, rash and severe persistent polyarthralgia in humans. Since there are currently no FDA licensed vaccines or antiviral therapies for CHIKV, the development of vaccine candidates is of critical importance. Historically, live...

Host Immune Response to Mosquito-Transmitted Chikungunya Virus Differs from That Elicited by Needle Inoculated Virus

Background Mosquito-borne diseases are a worldwide public health threat. Mosquitoes transmit viruses or parasites during feeding, along with salivary proteins that modulate host responses to facilitate both blood feeding and pathogen transmission. Understanding these earliest events in mosquito transmission of arboviruses by mosquitoes is essential for development and assessment...

Stability of Yellow Fever Virus under Recombinatory Pressure as Compared with Chikungunya Virus

Recombination is a mechanism whereby positive sense single stranded RNA viruses exchange segments of genetic information. Recent phylogenetic analyses of naturally occurring recombinant flaviviruses have raised concerns regarding the potential for the emergence of virulent recombinants either post-vaccination or following co-infection with two distinct wild-type viruses. To...

Impact of Wolbachia on Infection with Chikungunya and Yellow Fever Viruses in the Mosquito Vector Aedes aegypti

Incidence of disease due to dengue (DENV), chikungunya (CHIKV) and yellow fever (YFV) viruses is increasing in many parts of the world. The viruses are primarily transmitted by Aedes aegypti, a highly domesticated mosquito species that is notoriously difficult to control. When transinfected into Ae. aegypti, the intracellular bacterium Wolbachia has recently been shown to inhibit...

Endocytosis of Chikungunya Virus into Mammalian Cells: Role of Clathrin and Early Endosomal Compartments

Background The replicative cycle of chikungunya virus (CHIKV), an alphavirus that recently re-emerged in India and in Indian Ocean area, remains mostly unknown. The aim of the present study was to investigate the intracellular trafficking pathway(s) hijacked by CHIKV to enter mammalian cells. Methodology/Principal Findings Entry pathways were investigated using a variety of...

Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus

Background MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression in a variety of organisms, including insects, vertebrates, and plants. miRNAs play important roles in cell development and differentiation as well as in the cellular response to stress and infection. To date, there are limited reports of miRNA identification in mosquitoes...

Zika virus protection by a single low-dose nucleoside-modified mRNA vaccination

. Tam Yan-Jang Huang Dana Vanlandingham Veronica M. Holmes Harikrishnan Balachandran Sujata Sahu Michelle Lifton Stephen Higgs Scott E. Hensley Thomas D. Madden Michael J. Hope Katalin Karik? Sampa Santra

Aedes aegypti Saliva Alters Leukocyte Recruitment and Cytokine Signaling by Antigen-Presenting Cells during West Nile Virus Infection

West Nile virus (WNV) is transmitted during mosquito bloodfeeding. Consequently, the first vertebrate cells to contact WNV are cells in the skin, followed by those in the draining lymph node. Macrophages and dendritic cells are critical early responders in host defense against WNV infection, not just because of their role in orchestrating the immune response, but also because of...

A Single Mutation in Chikungunya Virus Affects Vector Specificity and Epidemic Potential

Chikungunya virus (CHIKV) is an emerging arbovirus associated with several recent large-scale epidemics. The 2005–2006 epidemic on Reunion island that resulted in approximately 266,000 human cases was associated with a strain of CHIKV with a mutation in the envelope protein gene (E1-A226V). To test the hypothesis that this mutation in the epidemic CHIKV (strain LR2006 OPY1) might...