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Human rhinovirus internal ribosome entry site element enhances transgene expression in transfected CHO-S cells

Contributions Tian-yun Wang took charge of the whole experiment, analyzed the data and helped to edit the manuscript. Yurong Chai wrote the manuscript and performed western blot. Meng-meng Ge performed cells ... -yun Wang OPEN Chinese hamster ovary (CHO) cells are mainly used for recombinant protein production. However, the unstable transgene expression and lower transgene copy numbers are the major issues need

Sequence architecture and sedimentary characteristics of a Middle Jurassic incised valley, western Sichuan depression, China

The Middle Jurassic Shaximiao Formation encompasses tens of meters of thick lowstand meandering valley (LMV) strata in the western Sichuan foreland basin. Ancient LMVs newly discovered in this area were further studied based on sequence stratigraphy and seismic sedimentology. The aim of the present study was to investigate the sedimentary characteristics, sequence architecture...

Impact of different promoters, promoter mutation, and an enhancer on recombinant protein expression in CHO cells

-fang Shang Chun-peng Zhao Tian-yun Wang OPEN In the present study, six commonly used promoters, including cytomegalovirus major immediateearly (CMV), the CMV enhancer fused to the chicken beta-actin ... Province (No. 18A350008). Author Contributions Tian-Yun Wang designed, analyzed the experiments?and revised the manuscript. Wen Wang and Yan-long Jia performed the experiments and wrote the manuscript. Yi

Matrix attachment region combinations increase transgene expression in transfected Chinese hamster ovary cells

for Yan-Long Jia in:Nature Research journals • PubMed • Google ScholarSearch for Tian-Yun Wang in:Nature Research journals • PubMed • Google Scholar Contributions Tian-Yun Wang designed, analyzed the ... -Nan Chen cultured cells. Competing interests The authors declare no competing financial interests. Corresponding authors Correspondence to Yan-Long Jia or Tian-Yun Wang. Rights and permissions This

Treating Colon Cancer Cells with FK228 Reveals a Link between Histone Lysine Acetylation and Extensive Changes in the Cellular Proteome

Molecular Diagnosis and Laboratory Medicine, Xinxiang 453003, ChinaTian-yun Wang & Yi-Chun Li AuthorsSearch for Tian-yun Wang in:Nature Research journals • PubMed • Google ScholarSearch for Yan-long Jia ... and Western blot. Y.-c.L. and J.-h.Z. co-wrote the manuscript. Competing interests The authors declare no competing financial interests. Corresponding author Correspondence to Tian-yun Wang

Preparation of DNA Ladder Based on Multiplex PCR Technique

Received 14 February 2010; Revised 26 April 2010; Accepted 11 May 2010 Academic Editor: Ashis Basu Copyright © 2010 Tian-Yun Wang et al. This is an open access article distributed under the Creative

Preparation of DNA Ladder Based on Multiplex PCR Technique

Received 14 February 2010; Revised 26 April 2010; Accepted 11 May 2010 Academic Editor: Ashis Basu Copyright © 2010 Tian-Yun Wang et al. This is an open access article distributed under the Creative

Simple, Time-Saving Dye Staining of Proteins for Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using Coomassie Blue

A fixation-free and fast protein-staining method for sodium dodecyl sulfate–polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol comprises staining and quick washing steps, which can be completed in 0.5 h. It has a sensitivity of 10 ng, comparable with that of conventional Coomassie Brilliant Blue G staining with phosphoric acid in the staining...

Genotypic variants at 2q33 and risk of esophageal squamous cell carcinoma in China: a meta-analysis of genome-wide association studies

Genome-wide association studies have identified susceptibility loci for esophageal squamous cell carcinoma (ESCC). We conducted a meta-analysis of all single-nucleotide polymorphisms (SNPs) that showed nominally significant P-values in two previously published genome-wide scans that included a total of 2961 ESCC cases and 3400 controls. The meta-analysis revealed five SNPs at...