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Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system

Background Clustered regularly interspaced short palindromic repeats interference (CRISPRi) is a recently developed powerful tool for gene regulation. In Escherichia coli, the type I CRISPR system expressed endogenously shall be easy for internal regulation without causing metabolic burden in compared with the widely used type II system, which expressed dCas9 as an additional...

A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome

Tianyuan Su in:Nature Research journals • PubMed • Google ScholarSearch for Fapeng Liu in:Nature Research journals • PubMed • Google ScholarSearch for Pengfei Gu in:Nature Research journals • PubMed • Google

A rapid and reliable strategy for chromosomal integration of gene(s) with multiple copies

ChinaPengfei Gu, Fan Yang, Tianyuan Su, Qian Wang, Quanfeng Liang & Qingsheng Qi AuthorsSearch for Pengfei Gu in:Nature Research journals • PubMed • Google ScholarSearch for Fan Yang in:Nature Research journals ... • PubMed • Google ScholarSearch for Tianyuan Su in:Nature Research journals • PubMed • Google ScholarSearch for Qian Wang in:Nature Research journals • PubMed • Google ScholarSearch for Quanfeng Liang