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7 papers found.
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Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna

: Hajime Watanabe. Resources: Tsuyoshi Nakanishi. Supervision: Yasuhiko Kato, Hajime Watanabe. Validation: Kerstin ToÈrner, Tomoaki Matsuura, Hajime Watanabe. Writing ± original draft: Kerstin ToÈrner

CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna

, Hajime Watanabe. Methodology: Takashi Nakanishi, Yasuhiko Kato, Hajime Watanabe. Project administration: Hajime Watanabe. Supervision: Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe. Validation

Sequence Conservation and Sexually Dimorphic Expression of the Ftz-F1 Gene in the Crustacean Daphnia magna

Identifying the genes required for environmental sex determination is important for understanding the evolution of diverse sex determination mechanisms in animals. Orthologs of Drosophila orphan receptor Fushi tarazu factor-1 (Ftz-F1) are known to function in genetic sex determination. In contrast, their roles in environmental sex determination remain unknown. In this study, we...

Heterodimeric TALENs induce targeted heritable mutations in the crustacean Daphnia magna

Tomoaki Matsuura Hajime Watanabe () Transcription activator-like effector nucleases (TALENs) are artificial nucleases harboring a customizable DNA-binding domain and a FokI nuclease domain. The high

CRISPR/Cas-Mediated Targeted Mutagenesis in Daphnia magna

The water flea Daphnia magna has been used as an animal model in ecology, evolution, and environmental sciences. Thanks to the recent progress in Daphnia genomics, genetic information such as the draft genome sequence and expressed sequence tags (ESTs) is now available. To investigate the relationship between phenotypes and the available genetic information about Daphnia, some...

Genomic Integration and Germline Transmission of Plasmid Injected into Crustacean Daphnia magna Eggs

The water flea, Daphnia, has been the subject of study in ecology, evolution, and environmental sciences for decades. Over the last few years, expressed sequence tags and a genome sequence have been determined. In addition, functional approaches of overexpression and gene silencing based on microinjection of RNAs into eggs have been established. However, the transient nature of...

Directed Evolution of Proteins through In Vitro Protein Synthesis in Liposomes

Directed evolution of proteins is a technique used to modify protein functions through “Darwinian selection.” In vitro compartmentalization (IVC) is an in vitro gene screening system for directed evolution of proteins. IVC establishes the link between genetic information (genotype) and the protein translated from the information (phenotype), which is essential for all directed...