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Application of loop-mediated isothermal amplification for malaria diagnosis during a follow-up study in São Tomé

Virgilio E do Rosario 6 I-Feng Lin 5 Men-Fang Shaio 0 1 4 0 The Anti-Malaria Team of Taiwan in Sao Tome and Principe , Sao Tome and Principe, Taipei , Taiwan 1 Department of Tropical Medicine, School of

Application of loop-mediated isothermal amplification for malaria diagnosis during a follow-up study in São Tomé

A reliable and simple test for the detection of malaria parasite is crucial in providing effective treatment and therapeutic follow-up, especially in malaria elimination programmes. A comparison of four methods, including nested polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) were used for the malaria diagnosis and treatment follow-up in São Tom...

Pre-elimination of malaria on the island of Príncipe

Background Plasmodium falciparum is the major species responsible for malaria transmission on the island of Príncipe, in the Republic of São Tomé and Príncipe (STP). Indoor residual spraying (IRS) has been intensively deployed on the island, since 2003. Other measures included intermittent preventive therapy (IPT), since 2004, as well as artemisinin-based therapy (ACT) and long...

Potential threat of malaria epidemics in a low transmission area, as exemplified by São Tomé and Príncipe

Background Plasmodium falciparum is the major cause of malaria infection in the island of São Tomé, in the Republic of São Tomé and Príncipe (STP), with an incidence of 40 - 50% before 2004. Since 2004, through the coordination of the Ministry of Health of STP and their Centro Nacional de Endemias (CNE), an integrated malaria control programme has been intensively deployed on the...

Genetic population structure of Anopheles gambiae in Equatorial Guinea

Background Patterns of genetic structure among mosquito vector populations in islands have received particular attention as these are considered potentially suitable sites for experimental trials on transgenic-based malaria control strategies. In this study, levels of genetic differentiation have been estimated between populations of Anopheles gambiae s.s. from the islands of...