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Shear Wave Elasticity Measurements of Three-Dimensional Cancer Cell Cultures Using Laser Speckle Contrast Imaging

Shear wave elastography (SWE) has been widely adopted for clinical in vivo imaging of tissue elasticity for disease diagnosis, and this modality can be a valuable tool for in vitro mechanobiology studies but its full potential has yet to be explored. Here we present a laser speckle contrast SWE system for noncontact monitoring the spatiotemporal changes of the extracellular...

A broadband photodetector based on Rhodamine B-sensitized ZnO nanowires film

figures independently. Ze Wen Liu is the corresponding author for this paper. All authors reviewed the manuscript. Additional Information Competing Interests: The authors declare that they have no

Salvianolic acid B inhibits mitochondrial dysfunction by up-regulating mortalin

Wen Liu supervised the entire study and were involved in design, acquisition of data, data analysis and interpretation and drafting the manuscript. All authors reviewed and approved the manuscript

The influence of postharvest UV-C treatment on anthocyanin biosynthesis in fresh-cut red cabbage

Red cabbage (Brassica oleracea L. var. capitata f. rubra DC.) is a fresh edible vegetable consumed globally that contains high levels of antioxidant compounds including anthocyanins. In this study, fresh-cut red cabbage was treated with different Ultraviolet-C (UV-C) dosages. Fifteen cyanidin derivatives were observed in UV-C treated fresh-cut red cabbage; four of these were...

Nanoscale zero-valent iron/persulfate enhanced upflow anaerobic sludge blanket reactor for dye removal: Insight into microbial metabolism and microbial community

This study investigated the efficiency of nanoscale zero-valent iron combined with persulfate (NZVI/PS) for enhanced degradation of brilliant red X-3B in an upflow anaerobic sludge blanket (UASB) reactor, and examined the effects of NZVI/PS on anaerobic microbial communities during the treatment process. The addition of NZVI (0.5 g/L) greatly enhanced the decolourization rate of...

Association between gut microbiota and diapause preparation in the cabbage beetle: a new perspective for studying insect diapause

Gut microbiota mediate the nutritional metabolism and play important roles in human obesity. Diapausing insects accumulate large fat reserves and develop obese phenotypes in order to survive unfavorable conditions. However, the possibility of an association between gut microbiota and insect diapause has not been investigated. We used the Illumina MiSeq platform to compare gut...

Levofloxacin loaded mesoporous silica microspheres/nano-hydroxyapatite/polyurethane composite scaffold for the treatment of chronic osteomyelitis with bone defects

, ChinaQi Wang, Cheng Chen, Wen Liu, Xiaoqiang He, Nian Zhou, Dongli Zhang & Wei HuangState Key Laboratory of Oncogenes and Related Genes School of Biomedical Engineering and Med-X Research Institute Shanghai ... AuthorsSearch for Qi Wang in:Nature Research journals • PubMed • Google ScholarSearch for Cheng Chen in:Nature Research journals • PubMed • Google ScholarSearch for Wen Liu in:Nature Research journals • PubMed

Arbuscular mycorrhizal fungi enhance soil carbon sequestration in the coalfields, northwest China

Carbon storage is affected by photosynthesis (Pn) and soil respiration (Rs), which have been studied extensively in natural and agricultural systems. However, the effects of Pn and Rs on carbon storages in the presence of arbuscular mycorrhizal fungi (AMF) in coalfields remain unclear. A field experiment was established in 2014 in Shendong coal mining subsidence area. The...

NEAP/DUSP26 suppresses receptor tyrosine kinases and regulates neuronal development in zebrafish

Expression of neuroendocrine-associated phosphatase (NEAP, also named as dual specificity phosphatase 26, [DUSP26]) is restricted to neuroendocrine tissues. We found that NEAP, but not its phosphatase-defective mutant, suppressed nerve growth factor (NGF) receptor TrkA and fibroblast growth factor receptor 1 (FGFR1) activation in PC12 cells upon NGF stimulation. Conversely...

The effects of manganese oxide octahedral molecular sieve chitosan microspheres on sludge bacterial community structures during sewage biological treatment

, Ministry of Education, Wuhan Textile University, Wuhan, 430073, ChinaFei PanEnvironmental Engineering Program, Department of Civil Engineering, Auburn University, Auburn, AL 36849, USAFei Pan, Wen Liu ...  & Dongye ZhaoSchool of Materials Science and Engineering, Wuhan Textile University, Wuhan 430073, ChinaXianze Yin AuthorsSearch for Fei Pan in:Nature Research journals • PubMed • Google ScholarSearch for Wen

Heat Shock Factor 1 Mediates Latent HIV Reactivation

HSF1, a conserved heat shock factor, has emerged as a key regulator of mammalian transcription in response to cellular metabolic status and stress. To our knowledge, it is not known whether HSF1 regulates viral transcription, particularly HIV-1 and its latent form. Here we reveal that HSF1 extensively participates in HIV transcription and is critical for HIV latent reactivation...

The chromatin scaffold protein SAFB1 localizes SUMO-1 to the promoters of ribosomal protein genes to facilitate transcription initiation and splicing

, Columbus, OH 43210 , USA 1 Present addresses: Hui-wen Liu, Oregon Health and Science University , Portland, OR 97239 , USA. Xiaoyan Guan, Florida State University , Tallahassee, FL , USA 2 Department of

Nanodroplet-Vaporization-Assisted Sonoporation for Highly Effective Delivery of Photothermal Treatment

Sonoporation refers to the use of ultrasound and acoustic cavitation to temporarily enhance the permeability of cellular membranes so as to enhance the delivery efficiency of therapeutic agents into cells. Microbubble-based ultrasound contrast agents are often used to facilitate these cavitation effects. This study used nanodroplets to significantly enhance the effectiveness of...

Magnetic-assisted triboelectric nanogenerators as self-powered visualized omnidirectional tilt sensing system

Microelectronics, Peking University, Beijing, China, 100871Mengdi Han, Xiao-Sheng Zhang, Xuming Sun, Bo Meng, Wen Liu & Haixia Zhang AuthorsSearch for Mengdi Han in:Nature Research journals • PubMed • Google ... • PubMed • Google ScholarSearch for Wen Liu in:Nature Research journals • PubMed • Google ScholarSearch for Haixia Zhang in:Nature Research journals • PubMed • Google Scholar Contributions M.H., X.S.Z. and

Structure-based Mechanistic Insights into Terminal Amide Synthase in Nosiheptide-Represented Thiopeptides Biosynthesis

, Renxiao Wang, Wen Liu & Chunyang CaoInstitute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai, 200031, ChinaTianlong ... Research journals • PubMed • Google ScholarSearch for Renxiao Wang in:Nature Research journals • PubMed • Google ScholarSearch for Wen Liu in:Nature Research journals • PubMed • Google ScholarSearch for

Improved lipids, diastolic pressure and kidney function are potential contributors to familial longevity: a study on 60 Chinese centenarian families

Xiao, Xiao-Qiong Chen, Yao-Wen Liu, Qin Yu, Li-Qin Yang, Xing-Li Yang, Ming-Xia Ge, Jian-Jun Jiang & Qing-Peng KongDepartment of Biochemistry and Molecular Biology, Hainan Medical College, Haikou 571199 ... , ChinaDong-Jing Yan & Wang-Wei CaiKIZ/CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming 650223, ChinaYong-Han He, Shao-Yan Pu, Fu-Hui Xiao, Xiao-Qiong Chen, Yao-Wen Liu

Promoters active in interphase are bookmarked during mitosis by ubiquitination

We analyzed modification of chromatin by ubiquitination in human cells and whether this mark changes through the cell cycle. HeLa cells were synchronized at different stages and regions of the genome with ubiquitinated chromatin were identified by affinity purification coupled with next-generation sequencing. During interphase, ubiquitin marked the chromatin on the transcribed...

Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes

SUMOylation of transcription factors and chromatin proteins is in many cases a negative mark that recruits factors that repress gene expression. In this study, we determined the occupancy of Small Ubiquitin-like MOdifier (SUMO)-1 on chromatin in HeLa cells by use of chromatin affinity purification coupled with next-generation sequencing. We found SUMO-1 localization on chromatin...

RMCE-ASAP: a gene targeting method for ES and somatic cells to accelerate phenotype analyses

In recent years, tremendous insight has been gained on p53 regulation by targeting mutations at the p53 locus using homologous recombination in ES cells to generate mutant mice. Although informative, this approach is inefficient, slow and expensive. To facilitate targeting at the p53 locus, we developed an improved Recombinase-Mediated Cassette Exchange (RMCE) method. Our...

Sequence and structure of the extrachromosomal palindrome encoding the ribosomal RNA genes in Dictyostelium

Ribosomal RNAs (rRNAs) are encoded by multicopy families of identical genes. In Dictyostelium and other protists, the rDNA is carried on extrachromosomal palindromic elements that comprise up to 20% of the nuclear DNA. We present the sequence of the 88 kb Dictyostelium rDNA element, noting that the rRNA genes are likely to be the only transcribed regions. By interrogating a...