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A Dual-Color Luciferase Assay System Reveals Circadian Resetting of Cultured Fibroblasts by Co-Cultured Adrenal Glands

In mammals, circadian rhythms of various organs and tissues are synchronized by pacemaker neurons in the suprachiasmatic nucleus (SCN) of the hypothalamus. Glucocorticoids released from the adrenal glands can synchronize circadian rhythms in other tissues. Many hormones show circadian rhythms in their plasma concentrations; however, whether organs outside the SCN can serve as...

An In Vitro Test to Screen Skin Sensitizers Using a Stable THP-1–Derived IL-8 Reporter Cell Line, THP-G8

Several studies have suggested that interleukin (IL)-8 can serve as a biomarker for discrimination of skin sensitizers from nonsensitizers. We established a stable THP-1–derived IL-8 reporter cell line, THP-G8, which harbors SLO and SLR luciferase genes under the control of IL-8 and glyceraldehyde 3-phosphate dehydrogenase promoters, respectively. After 6 h treatment with...

Concerted Evolution of Duplicated Control Regions within an Ostracod Mitochondrial Genome

Katsunori Ogoh 0 Yoshihiro Ohmiya 0 0 Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology , Ikeda, Osaka , Japan The luminescent marine

Simultaneous monitoring of independent gene expression patterns in two types of cocultured fibroblasts with different color-emitting luciferases

Background Luciferase assay systems enable the real-time monitoring of gene expression in living cells. We have developed a dual-color luciferase assay system in which the expression of multiple genes can be tracked simultaneously using green- and red-emitting beetle luciferases. We have applied the system to monitoring independent gene expressions in two types of cocultured...

Biogeography of Luminous Marine Ostracod Driven Irreversibly by the Japan Current

The biogeography of the luminous marine ostracod Vargula hilgendorfii, also called “Umihotaru,” shows that this organism may have arrived relatively recently on the Japanese islands during the final glacier period approximately 10,000 years ago. Phylogenetic relationships also strongly indicate that the Japan Current drove the Umihotaru ostracod northward. It is evident that the...

High-Sensitivity Real-Time Imaging of Dual Protein-Protein Interactions in Living Subjects Using Multicolor Luciferases

Networks of protein-protein interactions play key roles in numerous important biological processes in living subjects. An effective methodology to assess protein-protein interactions in living cells of interest is protein-fragment complement assay (PCA). Particularly the assays using fluorescent proteins are powerful techniques, but they do not directly track interactions because...

The influence of the region between residues 220 and 344 and beyond in Phrixotrix railroad worm luciferases green and red bioluminescence

To find the regions having a major influence on the bioluminescence spectra of railroad worm luciferases, we constructed new chimeric luciferases switching the fragments from residues 1–219 and from 220–545 between Phrixotrix viviani (PxvGR; λmax = 548 nm) green light‐emitting luciferase and Phrixothrix hirtus (PxhRE; λmax = 623 nm) red light‐emitting luciferases. The emission...