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Detection of Wolbachia in Aedes albopictus and Their Effects on Chikungunya Virus

, and Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, E-mail: . Indra Vythilingam and Yvonne A. L. Lim, Department of Parasitology, Faculty of Medicine

Frequencies distribution of dihydrofolate reductase and dihydropteroate synthetase mutant alleles associated with sulfadoxine–pyrimethamine resistance in Plasmodium falciparum population from Hadhramout Governorate, Yemen

Background Malaria in Yemen is mainly caused by Plasmodium falciparum and 25 % of the population is at high risk. Sulfadoxine–pyrimethamine (SP) had been used as monotherapy against P. falciparum. Emergence of chloroquine resistance led to the shift in anti-malarial treatment policy in Yemen to artemisinin-based combination therapy, that is artesunate (AS) plus SP as first-line...

Risk Factors for Enterovirus A71 Seropositivity in Rural Indigenous Populations in West Malaysia

Enterovirus A71 (EV-A71), which is transmitted by the fecal-oral route, causes hand, foot and mouth disease and, rarely, severe neurological complications. In Malaysia, the indigenous rural community (Orang Asli) has a high prevalence of parasitic diseases due to poor sanitation, water supply and hygiene practices. This cross-sectional study compared the seroepidemiology of EV...

Pathogenic waterborne free-living amoebae: An update from selected Southeast Asian countries

Data on the distribution of free-living amoebae is still lacking especially in Southeast Asian region. The aquatic environment revealed a high occurrence of free-living amoebae (FLA) due to its suitable condition and availability of food source, which subsequently causes infection to humans. A total of 94 water samples consisted of both treated and untreated from Laos (31...

First Molecular Characterization of Leishmania Species Causing Visceral Leishmaniasis among Children in Yemen

Visceral leishmaniasis (VL) is a debilitating, often fatal disease caused by Leishmania donovani complex; however, it is a neglected tropical disease. L. donovani complex comprises two closely related species, L. donovani that is mostly anthroponotic and L. infantum that is zoonotic. Differentiation between these two species is critical due to the differences in their...

Neglected Tropical Diseases among the Association of Southeast Asian Nations (ASEAN): Overview and Update

. Lim 0 Maureen M. Goodenow 0 Sazaly AbuBakar 0 0 Editor: Darren J. Gray, University of Queensland , AUSTRALIA The ten member states of the Association of Southeast Asian Nations (ASEAN) constitute an

Rapid Detection and Identification of Human Hookworm Infections through High Resolution Melting (HRM) Analysis

Background Hookworm infections are still endemic in low and middle income tropical countries with greater impact on the socioeconomic and public health of the bottom billion of the world's poorest people. In this study, a real-time polymerase chain reaction (PCR) coupled with high resolution melting-curve (HRM) analysis was evaluated for an accurate, rapid and sensitive tool for...

Antibody Prevalence and Factors Associated with Exposure to Orientia tsutsugamushi in Different Aboriginal Subgroups in West Malaysia

. Lim 0 Romano Ngui 0 David H. Walker, University of Texas Medical Branch, United States of America 0 1 Tropical Infectious Diseases Research and Education Centre (TIDREC), Department of Medical

New insights into the genetic diversity of Schistosoma mansoni and S. haematobium in Yemen

Background Human schistosomiasis is a neglected tropical disease of great importance that remains highly prevalent in Yemen, especially amongst rural communities. In order to investigate the genetic diversity of human Schistosoma species, a DNA barcoding study was conducted on S. mansoni and S. haematobium in Yemen. Methods A cross-sectional study was conducted to collect urine...

Prevalence and Associated Factors of Schistosomiasis among Children in Yemen: Implications for an Effective Control Programme

Background Schistosomiasis, one of the most prevalent neglected tropical diseases, is a life-threatening public health problem in Yemen especially in rural communities. This cross-sectional study aims to determine the prevalence and associated risk factors of schistosomiasis among children in rural Yemen. Methods/Findings Urine and faecal samples were collected from 400 children...

High Diversity of Cryptosporidium Subgenotypes Identified in Malaysian HIV/AIDS Individuals Targeting gp60 Gene

Background Currently, there is a lack of vital information in the genetic makeup of Cryptosporidium especially in developing countries. The present study aimed at determining the genotypes and subgenotypes of Cryptosporidium in hospitalized Malaysian human immunodeficiency virus (HIV) positive patients. Methodology/Principal Findings In this study, 346 faecal samples collected...

Epidemiological and Genetic Data Supporting the Transmission of Ancylostoma ceylanicum among Human and Domestic Animals

Background Currently, information on species-specific hookworm infection is unavailable in Malaysia and is restricted worldwide due to limited application of molecular diagnostic tools. Given the importance of accurate identification of hookworms, this study was conducted as part of an ongoing molecular epidemiological investigation aimed at providing the first documented data on...

Molecular Identification of Human Hookworm Infections in Economically Disadvantaged Communities in Peninsular Malaysia

, Malaysia 1 Department of Parasitology, Faculty of Medicine, University of Malaya , Kuala Lumpur Malaysia 2 Authors' addresses: Romano Ngui, Lee Soo Ching, Tan Tiong Kai, Muhammad Aidil Roslan, and Yvonne A ... . L. Lim, Department of Parasitology, Faculty of Medicine, University of Malaya , Kuala Lumpur , Malaysia Species identification of human hookworm infections among eight communities in rural areas of

Epidemiology of Intestinal Polyparasitism among Orang Asli School Children in Rural Malaysia

Background This cross-sectional study aimed to investigate the current prevalence and risk factors associated with intestinal polyparasitism (the concurrent infection with multiple intestinal parasite species) among Orang Asli school children in the Lipis district of Pahang state, Malaysia. Methods/Principal findings Fecal samples were collected from 498 school children (50.6...

Factors Associated with High Prevalence of Intestinal Protozoan Infections among Patients in Sana'a City, Yemen

Background Intestinal protozoan diseases in Yemen are a significant health problem with prevalence ranging from 18% to 27%. The present study is a cross-sectional study aimed at determining the factors associated with the high prevalence of intestinal protozoan infections among patients seeking health care in Sana'a City, the capital of Yemen. Methodology/Principal Findings Stool...

Seroprevalence and Sources of Toxoplasmosis among Orang Asli (Indigenous) Communities in Peninsular Malaysia

, University of Malaya , 50603 Kuala Lumpur , Malaysia 3 Authors' addresses: Romano Ngui, Yvonne A. L. Lim, Noor Farah Hani Amir,Veeranoot Nissapatorn, and Rohela Mahmud, Department of Parasitology, Faculty of

Mitochondrial DNA Markers Reveal High Genetic Diversity but Low Genetic Differentiation in the Black Fly Simulium tani Takaoka & Davies along an Elevational Gradient in Malaysia

The population genetic structure of Simulium tani was inferred from mitochondria-encoded sequences of cytochrome c oxidase subunits I (COI) and II (COII) along an elevational gradient in Cameron Highlands, Malaysia. A statistical parsimony network of 71 individuals revealed 71 haplotypes in the COI gene and 43 haplotypes in the COII gene; the concatenated sequences of the COI and...

Prevalence and Risk Factors of Intestinal Parasitism in Rural and Remote West Malaysia

Background Intestinal parasitic infections (IPIs) have a worldwide distribution and have been identified as one of the most significant causes of illnesses and diseases among the disadvantaged population. In Malaysia, IPIs still persist in some rural areas, and this study was conducted to determine the current epidemiological status and to identify risk factors associated with...

Comparative Study on Waterborne Parasites between Malaysia and Thailand: A New Insight

, Dasmarin as, The Philippines; School of Science and Technology, Centro Escolar University , Manila , The Philippines 2 Authors' addresses: Thulasi Kumar, Subashini Onichandran, Yvonne A. L. Lim , Init Ithoi