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Surveillance of Live Poultry Markets for Low Pathogenic Avian Influenza Viruses in Guangxi Province, Southern China, from 2012–2015

Institute, 51 You Ai North Road, Nanning, Guangxi, 530001, ChinaSisi Luo, Zhixun Xie, Zhiqin Xie, Liji Xie, Li Huang, Jiaoling Huang, Xianwen Deng, Tingting Zeng, Sheng Wang, Yanfang Zhang & Jiabo Liu ... AuthorsSearch for Sisi Luo in:Nature Research journals • PubMed • Google Scholar Search for Zhixun Xie in:Nature Research journals • PubMed • Google Scholar Search for Zhiqin Xie in:Nature Research journals

Development of a GeXP-multiplex PCR assay for the simultaneous detection and differentiation of six cattle viruses

Foot-and-mouth disease virus (FMDV), Bluetongue virus (BTV), Vesicular stomatitis Virus (VSV), Bovine viral diarrheal (BVDV), Bovine rotavirus (BRV), and Bovine herpesvirus 1 (IBRV) are common cattle infectious viruses that cause a great economic loss every year in many parts of the world. A rapid and high-throughput GenomeLab Gene Expression Profiler (GeXP) analyzer-based...

Simultaneous detection of eight immunosuppressive chicken viruses using a GeXP analyser-based multiplex PCR assay

Background Immunosuppressive viruses are frequently found as co-infections in the chicken industry, potentially causing serious economic losses. Because traditional molecular biology methods have limited detection ability, a rapid, high-throughput method for the differential diagnosis of these viruses is needed. The objective of this study is to develop a GenomeLab Gene...

Reverse-transcription, loop-mediated isothermal amplification assay for the sensitive and rapid detection of H10 subtype avian influenza viruses

Background The H10 subtype avian influenza viruses (H10N4, H10N5 and H10N7) have been reported to cause disease in mammals, and the first human case of H10N8 subtype avian influenza virus was reported in 2013. Recently, H10 subtype avian influenza viruses (AIVs) have been followed more closely, but routine diagnostic tests are tedious, less sensitive and time consuming, rapid...

Role of phosphatidylinositol 3-kinase (PI3K) and Akt1 kinase in porcine reproductive and respiratory syndrome virus (PRRSV) replication

We have previously reported that inhibition of phosphatidylinositol 3-kinase (PI3K) reduces porcine reproductive and respiratory syndrome (PRRSV) replication. Here, we further investigate the mechanism by which PI3K inhibition affects virus replication and the role of Akt1 kinase in virus replication. We found that PI3K inhibition reduced viral gene transcription by approximately...

A duplex real-time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae

Background Infectious arthritis in broilers represents an economic and health problem, resulting in severe losses due to retarded growth and downgrading at the slaughterhouse. The most common agents associated with cases of infectious arthritis in poultry are avian reovirus (ARV) and Mycoplasma synoviae (MS). The accurate differentiation and rapid diagnosis of ARV and MS are...

A loop-mediated isothermal amplification assay for the visual detection of duck circovirus

Background Duck circovirus (DuCV) infection in farmed ducks is associated with growth problems or retardation syndromes. Rapid identification of DuCV infected ducks is essential to control DuCV effectively. Therefore, this study aims to develop of an assay for DuCV to be highly specific, sensitive, and simple without any specialized equipment. Methods A set of six specific...

Ultrasensitive Electrochemical Immunoassay for Avian Influenza Subtype H5 Using Nanocomposite

may have a promising potential in clinical applications. Conceived and designed the experiments: Zhixun Xie JH. Performed the experiments: JH SL Zhiqin Xie LX JL YP XD QF. Analyzed the data: Zhixun Xie ... JH. Contributed reagents/materials/analysis tools: Zhixun Xie JH SL Zhiqin Xie LX JL YP XD QF. Wrote the paper: Zhixun Xie JH. 1. Wang RH , Wang Y , Lassiter K , Li YB , Billy H , et al. ( 2009

Reverse transcription loop-mediated isothermal amplification assay for rapid detection of Bovine Rotavirus

Background Bovine rotavirus (BRV) infection is common in young calves. This viral infection causes acute diarrhea leading to death. Rapid identification of infected calves is essential to control BRV successfully. Therefore development of simple, highly specific, and sensitive detection method for BRV is needed. Results A reverse transcription loop-mediated isothermal...

Visual detection of H3 subtype avian influenza viruses by reverse transcription loop-mediated isothermal amplification assay

Background Recent epidemiological investigation of different HA subtypes of avian influenza viruses (AIVs) shows that the H3 subtype is the most predominant among low pathogenic AIVs (LPAIVs), and the seasonal variations in isolation of H3 subtype AIVs are consistent with that of human H3 subtype influenza viruses. Consequently, the development of a rapid, simple, sensitive...