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26 papers found.
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Effective and precise adenine base editing in mouse zygotes

, Hongwei Sun, Xiya Zhang, Xiaowei Xie, Jinran Zhang, Yaofu Bai, Xueling Ouyang, Shengyao Zhi, Yuanyan Xiong, Wenbin Ma, Dan Liu, Junjiu Huang and Zhou Songyang declare that they have no conflict of interest

Systematic analysis of human telomeric dysfunction using inducible telosome/shelterin CRISPR/Cas9 knockout cells

CRISPR/Cas9 technology enables efficient loss-of-function analysis of human genes using somatic cells. Studies of essential genes, however, require conditional knockout (KO) cells. Here, we describe the generation of inducible CRISPR KO human cell lines for the subunits of the telosome/shelterin complex, TRF1, TRF2, RAP1, TIN2, TPP1 and POT1, which directly interact with...

SmedOB1 is Required for Planarian Homeostasis and Regeneration

McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, USADan Liu & Zhou Songyang AuthorsSearch for Shanshan Yin in:Nature Research journals • PubMed • Google ... in:Nature Research journals • PubMed • Google ScholarSearch for Zhou Songyang in:Nature Research journals • PubMed • Google Scholar Contributions Y.H. and Z.S.conceived and designed the experiments. S.Y., Y.H

Correction of β-thalassemia mutant by base editor in human embryos

, Xiya Zhang, Ying Sun, Yuanyan Xiong, Wenbin Ma, Yongxiang Liu, Yali Wang, Jianpei Fang, Dan Liu, Zhou Songyang, Canquan Zhou, and Junjiu Huang declare that they have no conflict of interest. All

Effective gene editing by high-fidelity base editor 2 in mouse zygotes

Zhang, Yuxi Chen, Chenhui Ding, Yuanyan Xiong, Wenbin Ma, Dan Liu, Junjiu Huang, and Zhou Songyang declare that they have no conflict of interest. All institutional and national guidelines for the care

CCSI: a database providing chromatin–chromatin spatial interaction information

Distal regulatory elements have been shown to regulate gene transcription through spatial interactions, and single nucleotide polymorphisms (SNPs) are linked with distal gene expression by spatial proximity, which helps to explain the causal role of disease-associated SNPs in non-coding region. Therefore, studies on spatial interactions between chromatin have created a new avenue...

Specific Tandem 3'UTR Patterns and Gene Expression Profiles in Mouse Thy1+ Germline Stem Cells

A recently developed strategy of sequencing alternative polyadenylation (APA) sites (SAPAS) with second-generation sequencing technology can be used to explore complete genome-wide patterns of tandem APA sites and global gene expression profiles. spermatogonial stem cells (SSCs) maintain long-term reproductive abilities in male mammals. The detailed mechanisms by which SSCs self...

TeloPIN: a database of telomeric proteins interaction network in mammalian cells

Interaction network surrounding telomeres has been intensively studied during the past two decades. However, no specific resource by integrating telomere interaction information data is currently available. To facilitate the understanding of the molecular interaction network by which telomeres are associated with biological process and diseases, we have developed TeloPIN...

Telomere regulation in pluripotent stem cells

; MEF, mouse embryonic fibroblast; PML, promyelocytic leukemia; PSCs, pluripotent stem cells. COMPLIANCE WITH ETHICS GUIDELINES Yan Huang, Puping Liang, Dan Liu, Junjiu Huang and Zhou Songyang declare

CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo

Mammalian spermatogenesis is a highly regulated multistage process of sperm generation. It is hard to uncover the real function of a testis specific gene in vitro since the in vitro model is not yet mature. With the development of the CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated 9) system, we can now rapidly generate knockout mouse...

Cold-inducible RNA-binding protein CIRP/hnRNP A18 regulates telomerase activity in a temperature-dependent manner

The telomerase is responsible for adding telomeric repeats to chromosomal ends and consists of the reverse transcriptase TERT and the RNA subunit TERC. The expression and activity of the telomerase are tightly regulated, and aberrant activation of the telomerase has been observed in >85% of human cancers. To better understand telomerase regulation, we performed...

Human cells lacking coilin and Cajal bodies are proficient in telomerase assembly, trafficking and telomere maintenance

The RNA component of human telomerase (hTR) localizes to Cajal bodies, and it has been proposed that Cajal bodies play a role in the assembly of telomerase holoenzyme and telomerase trafficking. Here, the role of Cajal bodies was examined in Human cells deficient of coilin (i.e. coilin-knockout (KO) cells), in which no Cajal bodies are detected. In coilin-KO cells, a normal level...

Fam118B, a newly identified component of Cajal bodies, is required for Cajal body formation, snRNP biogenesis and cell viability

Cajal bodies are specialized and dynamic compartments in the nucleus that are involved in the biogenesis of small nuclear ribonucleoproteins (snRNPs). Because of the dynamic and varied roles of Cajal bodies, it is of great interest to identify the components of Cajal bodies to better understand their functions. We performed a genome-wide screen to identify proteins that...

Zebrafish as a Model System to Study the Physiological Function of Telomeric Protein TPP1

Telomeres are specialized chromatin structures at the end of chromosomes. Telomere dysfunction can lead to chromosomal abnormalities, DNA damage responses, and even cancer. In mammalian cells, a six-protein complex (telosome/shelterin) is assembled on the telomeres through the interactions between various domain structures of the six telomere proteins (POT1, TPP1, TIN2, TRF1...

CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes

Bai, Zhou Songyang, Wenbin Ma, Canquan Zhou, and Junjiu Huang declare that they have no conflict of interest. This study conformed to ethical standards of Helsinki Declaration and national legislation

The telosome/shelterin complex and its functions

The telomeres that cap the ends of eukaryotic chromosomes serve a dual role in protecting the chromosome ends and in intracellular signaling for regulating cell proliferation. A complex of six telomere-associated proteins has been identified - the telosome or shelterin complex - that is crucial for both the maintenance of telomere structure and its signaling functions.