Nature Protocols

List of Papers (Total 220)

Report Approval for Transcranial Electrical Stimulation (RATES): expert recommendation based on a Delphi consensus study

Transcranial electrical stimulation (tES) has gained substantial momentum as a research and therapeutic tool; however, it suffers from challenges related to reproducibility and quality assessment due to the absence of standardized reporting practices. Here we aim to develop a comprehensive and consensus-based checklist for conducting and reporting tES studies to enhance the...

Expansion of human pluripotent stem cell–induced nephron progenitor cells (iNPCs) and the generation of nephron organoids from iNPCs

Nephron progenitor cells (NPCs) have a central role in kidney organogenesis: they self-renew and differentiate into nephrons, the functional units of the kidney. Human pluripotent stem cells (hPSCs) can transiently produce induced nephron progenitor-like cells (iNPCs), which then differentiate into nephron organoids. Here, we describe a protocol to purify and expand the hPSC...

OBSERVE: guidelines for the refinement of rodent cancer models

Existing guidelines on the preparation (Planning Research and Experimental Procedures on Animals: Recommendations for Excellence (PREPARE)) and reporting (Animal Research: Reporting of In Vivo Experiments (ARRIVE)) of animal experiments do not provide a clear and standardized approach for refinement during in vivo cancer studies, resulting in the publication of generic...

The ClusPro AbEMap web server for the prediction of antibody epitopes

Antibodies play an important role in the immune system by binding to molecules called antigens at their respective epitopes. These interfaces or epitopes are structural entities determined by the interactions between an antibody and an antigen, making them ideal systems to analyze by using docking programs. Since the advent of high-throughput antibody sequencing, the ability to...