Identifying the microbial taxa that structure assemblages in response to local disturbances along the Chilean Patagonian coast is critical for understanding community reorganization and ecological risk in this vulnerable marine environment. Here, we examined benthic microbial interactions across sixteen sites spanning 42–44°S in the Inner Sea of Chiloé and adjacent fjord systems...
Perianal abscesses are common in colorectal surgery, requiring surgical drainage and wound care. Diabetic patients often experience delayed wound healing and increased infection risk due to systemic factors and altered microbiota. This study investigated the postoperative wound microbiota in diabetic patients with perianal abscesses using 16s rRNA sequencing, aiming to identify...
Despite significant global efforts to control HCV infection, there has been no observed decline in the incidence of new infections or mortality. The asymptomatic and silently progressing nature of HCV infection delays the response to potential treatments. Hence, there is a need for rapid diagnostic techniques that enable the early detection and treatment of the disease before it...
Catheter-associated urinary tract infections (CAUTIs) are a major healthcare challenge due to bacterial biofilm formation, which protects pathogens from antibiotics and host immune responses. Three phenotypic biofilm detection methods—Microplate assay, Tube Method, and Modified Congo Red Agar (MCRA)— were compared using bacterial isolates from catheter tips and urine samples. The...
Method suitability for microbial limit tests during microbiological quality control (QC) can be a complicated process that requires multiple steps of optimization and plays a central role in ensuring reliable QC results. When antimicrobial activity of a given product cannot be neutralized, it is assumed that the inhibited microorganism is not present in the product, as specified...
Differences in the gut microbiome between lean and obese individuals, even twins, have been recognized for over a decade. The causative role of the microbiome in obesity is known from both mouse and human studies. In parallel, the gut microbiome has been implicated in the most common complications of pregnancy, including preterm birth, gestational diabetes mellitus, and...
Early gastrointestinal microbiota establishment is crucial for host metabolism and immune development, with delivery mode and breastfeeding playing key roles. Vaginal delivery promotes colonization by maternal vaginal and gut microbes, while Caesarean section delivery leads to exposures of environmental and skin-derived microbiota. Although maternal contributions have been...
The intertidal zone where mangroves inhabit is unique for both plants and microbes. The periodic seawater tides have allowed only a very small portion of woody plants to shift as mangroves. However, the understanding of how seawater tides shape bacterial community in mangrove sediment is not yet comprehensive. By comparing mangrove sediment in low-tidal zones to land soil in...
Antimicrobial resistance (AMR) poses a significant threat to public health, with the Global Action Plan advocating, among others, for strengthening AMR knowledge and evidence base through surveillance and research and optimizing the use of antimicrobial medicines in human and animal health. However, due to inadequate healthcare infrastructure in many resource-constrained...
Phytoplankton play a vital role in global primary production and carbon fixation in marine ecosystems. A comprehensive understanding of their distribution patterns and assembly mechanisms under different nutrient conditions is essential for phytoplankton ecology. However, research in this field remains insufficient. This study used environmental DNA (eDNA) technology targeting...
The dysbiosis of gut microbiome is known to have a significant impact on the progression of sever acute pancreatitis (SAP). This study intends to investigate the alteration of gut microbiome in SAP patients during hospitalization and its association with mortality. Bacterial and fungal compositions of the fecal microbiota were determined via 16S and ITS1 sequencing, respectively...
Urothelial carcinoma (UC) is the most common malignant tumor of the urinary system, characterized by high incidence and recurrence rates, posing a serious threat to human health. While previous studies have linked urinary microbiota alterations to bladder cancer, little is known about the broader spectrum of UC subtypes or their clinical implications. To address this gap, we...
Plant microbiome is a very wide research area playing a major role in agriculture and plant health. This study investigates the interactions between root-associated bacterial communities and soil physico-chemical characteristics of four Moroccan desert plant species: Lavandula coronopifolia Poir. (n = 20), Lycium intricatum Boiss. (n = 20), Nitraria retusa Asch. (n = 20) and...
Viruses are traditionally viewed as intracellular parasites that exploit host resources to propagate, often at the host’s expense. However, emerging evidence suggests more nuanced interactions, including potential mutualism. The L-A double-stranded RNA (dsRNA) virus, a non-lytic, cytoplasmic virus commonly found in Saccharomyces cerevisiae, presents an intriguing case, as it...
Bloodstream infections (BSI) related mortality rates are increasing worldwide making it a medical emergency. This study has evaluated a new method for direct detection of pathogens and to perform direct antimicrobial susceptibility testing from the positive flagged blood culture. Early diagnosis and prompt treatment with appropriate antibiotics is the utmost need. A 30-minute...
Osteonecrosis of the femoral head usually occurred after glucocorticoid (GC) therapy. The gut microbiota was considered an indispensable part of maintaining human health, and the concept of the gut microbiota -bone axis was also proposed. In our study, we aimed to elucidate the link between steroid-induce osteonecrosis of the femoral head (SIONFH) and the gut microbiota; Methods...
Transport stress poses a major challenge to the livestock industry. Studies have shown that transportation stress can cause depression, elevated body temperature and dysbiosis of intestinal microbiota in meat goats, and in severe cases, even lead to death of the animals, which in turn affects the economic efficiency of the farming industry. However, the changes in blood...
Surgical site infections (SSIs) remain a significant cause of postoperative morbidity and are often associated with multidrug-resistant (MDR) organisms. This study aimed to isolate, identify, and evaluate the antibiotic susceptibility patterns of bacterial pathogens from postoperative wound infections in a tertiary care center. A postoperative cross-sectional study conducted on...
Ethiopia harbors a large cattle population that significantly contributes to the national economy through meat and dairy production. However, bacterial contamination of raw meat and antimicrobial resistance present serious public health challenges. This study aimed to isolate zoonotic bacterial pathogens from raw cattle meat and assess their antibiotic resistance profiles in the...
Dandruff represents a complex, multifactorial disorder characterized by impaired barrier function, altered lipid composition, and microbial imbalance. The principal pathogenic mechanism, however, involves excessive colonization by Malassezia species, notably M. furfur, M. restricta and M. globosa, on the scalp. Standard antifungal options often lead to undesirable effects...
Antibiotic resistance poses a significant threat to global food safety, particularly in unpasteurised dairy products. In the locality of Bwegera (South Kivu, DRC), livestock management practices promote the emergence of resistant bacterial strains, constituting a risk to public health. This study aimed to assess the resistance profile of enteropathogenic bacteria isolated from...
To characterize the reproductive tract microbiota in patients with ovarian chocolate cysts (CC) and adenomyosis (AM) compared to controls (NC), and investigate the in vitro effects of representative differential bacteria on human endometrial stromal cells (T-HESC). Microbiota profiles from the cervical canal, posterior fornix, ascites, and endometrium of 20 CC, 20 AM, and 20 NC...
Pulmonary mucormycosis (PM), a life-threatening infection caused by Mucorales, exhibits high mortality. Comprehensive data integrating clinical profiling, antifungal susceptibility, and biofilm formation ability are limited. This retrospective study characterized 26 adult PM patients at a Beijing tertiary-care hospital. Data on demographics, underlying diseases, co-infections...
Accurately identifying genes responsible for specific functions is a cornerstone of biological research, but current methods are often limited to single-species analyses. Here, we present a novel method, called Genomic and Phenotype-based machine learning for Gene Identification (GPGI), that leverages large-scale, cross-species genomic and phenotypic data for functional gene...
The Alternaria fungus represents a significant threat to grain storage safety due to its production of mycotoxins, which pose serious risks to human health. This study explores the toxin profile of 153 stored wheat samples collected from five distinct regions in Xizang , analyzed by ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Our findings...