Ancient DNA from fossil equids: a milestone in palaeogenetics

Thackeray News & Views Ancient DNA from fossil equids: A milestone in palaeogenetics Authors: J. Francis Thackeray1 Affiliation: 1 Institute for Human Evolution, University of the Witwatersrand, South Africa email: Francis.thackeray@wits. ac.za South African Journal of Science Article #111 Postal address: Institute for Human Evolution, University of the Witwatersrand, PO WITS, Johannesburg 2050, South Africa How to cite this article: Thackeray JF. Ancient DNA from fossil equids: A milestone in palaeogenetics. S Afr J Sci. 2010;106(1/2), Art. #111, 2 pages. DOI: 10.4102/sajs. v106i1/2.111 This article is available at: http://www.sajs.co.za © 2010. The Authors. Licensee: OpenJournals Publishing. This work is licensed under the Creative Commons Attribution License. 4 S Afr J Sci The family Equidae includes a diversity of extant species in the single genus Equus, notably horses (E. caballus and E. przewalskii) and a group called the non-caballines (which includes both Asian and African asses, as well as African zebras). The fossil record, however, shows even more remarkable diversity: within the past 5 million years at least 12 equid genera have been documented, including Hipparion, which is known from Plio-Pleistocene deposits in the Cradle of Humankind World Heritage Site in South Africa. A linear evolutionary sequence from a hyracotherian ancestor was proposed by Othaniel Marsh and championed by Thomas Huxley, but the evolution of equids within the past 55 million years is now recognized as being much more complex. Palaeontologists such as Basil Cooke, Rufus Churcher, Vera Eisenmann and Alan Gentry have generally relied on morphology and measurements for purposes of addressing the taxonomy of southern African Equidae, as others have done for equids from other areas. More recently the potential of genetic data has been demonstrated, most remarkably in a study published in December by Orlando et al.1 In the 1980s, Higuchi et al.2,3 succeeded in isolating mitochondrial DNA from the tissue of a museum specimen of the partially striped South African Cape ‘quagga’, which became extinct in the 19th century. The genetic results stimulated a great deal of discussion as to whether the Cape quagga was distinct at the species level from the more completely striped Burchell’s zebra.4,5 Genetic techniques to analyse modern and ancient DNA have improved dramatically within the past 25 years. A striking demonstration of this was Orlando et al.’s successful recovery of a DNA from 22 of 35 teeth of Equus, dating to the Late Pleistocene or Holocene. Four of the teeth which were successfully analysed for ancient DNA were attributed to E. capensis, a species which has been thought to have become extinct in South Africa in the Late Quaternary. Other fossil equid specimens in the analysis were from sites in Europe, Asia and the American continents. The late Reinhold Rau, taxidermist at the South African Museum (now part of the Iziko Museums of Cape Town), was delighted by the initial mitochondrial DNA analyses because he had observed a gradation in the degree of striping from north to south, which suggested clinal variation without a clear boundary between two taxa.6,7 Similar sentiments were expressed by Thackeray4,5 on the basis of variation in the entoflexid of a lower premolar (P₄). He used modern museum specimens of equids from the Transvaal Museum (Northern Flagship Institution), the Natural History Museum (London) and the American Museum of Natural History (New York) for comparative purposes, as well as fossil equid specimens from Wonderwerk Cave, near Kuruman in the Northern Cape Province of South Africa, which has a long Quaternary sequence. Wonderwerk is particularly interesting, as it is situated at the northern limit of the distribution of the partially striped ‘quagga’ and close to the southern limit Source: Photograph taken by Frederick York and Frank Haes. Downloaded from http://en.wikipedia.org/wiki/Quagga A partially striped quagga (Equus quagga quagga) photographed alive in 1870 in the Regent’s Park Zoo in London Vol. 106 No. 1/2 Page 1 of 2 http://www.sajs.co.za News & Views of that of the plains zebra. The latter was formerly classified as E. burchelli, but was reclassified as E. q. burchelli, distinct at a subspecies level from the historically extinct E. q. quagga on the basis of mitochondrial DNA analyses by Higuchi et al.2,3 4. Thackeray JF. Zebras from Wonderwerk Cave, northern Cape Province: Attempts to distinguish Equus burchelli and E. quagga. S Afr J Sci. 1988;84:99−101. 5. Thackeray JF. Morphometric, palaeoecological and taxonomic considerations of southern African zebras: Attempts to distinguish the quagga. S Afr J Sci. 1997;93:89−93. 6. Rau RE. Revised list of the preserved material of the extinct Cape Colony Quagga, Equus quagga quagga (Gmelin). Ann S Afr Mus. 1974;65:41−86. 7. Rau RE. Additions to the revised list of preserved material of the extinct Cape Colony Quagga and notes on the relationship and distribution of southern Plains Zebras. Annals of the South African Museum, 1978;77:27−45. 8. Churcher CS. The extinct Cape zebra. Sagittarius. 1986;1:4−5. 9. Thackeray JF, Brink JS. Damaliscus niro horns from Wonderwerk Cave and other Pleistocene sites: Morphological and chronological considerations. Palaeontol Afr. 2004;40:89−93. 10. Thackeray JF, Lee-Thorp JA. Isotopic analysis of equid teeth from Wonderwerk Cave, northern Cape Province, South Africa. Palaeogeogr palaeoclimateol palaeoecol. 1992;99:141−150. The extraordinary ancient DNA results by Orlando et al.1 were published in a recent issue of the Proceedings of the National Academy of Sciences. They were obtained from two separate laboratories: one in Lyon in France under Ludovic Orlando himself and the other, at Adelaide at the Australian Centre for Ancient DNA, under the direction of Alan Cooper. The paper was co-authored by a team of 21 other researchers from many parts of the world. After checking and controlling for contamination, the team used the DNA results to explore phylogenetic relationships. The DNA clearly separated Old World non-caballine equids from caballine and New World horses. In the case of Africa, the four specimens attributed to E. capensis (three from Wonderwerk Cave, and one from Glen Craig Shelter near Port Elizabeth) grouped closely with specimens attributed to E. q burchelli and E. q quagga. Specimens of the latter taxon were obtained from the Iziko Museums (South Africa), the Yale Peabody Museum (New Haven, USA), as well as the Darmstadt and Berlin museums in Germany. Equus greyvi from Ethiopia and Kenya appears to group more closely with Asian asses than with the South African zebras. The mountain zebra (E. hartmannae) does not group with E q. quagga or E. q. burchelli. Instead, the latter two taxa are more closely related to each other than either is to the mountain zebra, E. hartmannae. These exciting results constitute an important milestone in the (...truncated)