Improved peptide function from random mutagenesis over short peptide ‘windows’

Protein Engineering vol.2 no.5 p.393, 1989 Corrigenda Primary and predicted secondary structures of the caseins in relation to their biological functions by C.Holt and L.Sawyer Protein Engineering, 2, 251-259 (1988) The second part of Figure 4 was omitted from the above paper. The complete figure and legend are published below. Guinea pig Rat Cow K K L L I L T C L V A H K L L I L T C L V A H K L L I L T C L V A S|A|V|A|«|P|K Improved peptide function from random mutagenesis over short peptide 'windows' by I.S.Dunn, R.Cowan and P.A.Jennings Protein Engineering, 2, 283-291 (1988) In Table I, mutant B/P9 under column heading 'Site B' is shown as NYTVA. It should be KYTVA and the corresponding double window mutant in Table II should also be shown as KYTVA. F P F R H T E L F Q T Q R G G R R N A Y S S Q T Q Q E P I K H Q G L P Q Secondary structure S S S S S S S E E R L I I E H I l I F D S g i N S S S E E Q E I V K Q P K Y L S L N E E F V N N L N R O . R E V L N E N L L R F F V A P F P E - Secondary structure Guinea pig Rat COW Guinta pig Rat Cow a a a a a a a E L Q R K Q S E K I E L L T E Q D N E I V F G K E K V N E L - KE I I S EST E Q R E A - I I THD SSA EEQ A T A S A Q E D S S S I D IG SE S T E D Q A M E D I K Q H E A S S I S S S E S I Secondary structure Guinea pig Rat Cow V V P K N S K D A I P S I V P N A I P Q E D A L T T Y Q Q A 1 0 1 N|I A N R E I L L I I C TL I Q K E D V P S E R Y L G S V Y L Secondary structure Guinea pig Rat Cow E Q L E Q L Q R Q IK I S E Q L Q L L Q Q H S L A O Q A S L A Q Q A S L A Q Q A Secondary structure Guinea p i g Rat LLAQQPSLAQQAALAQQASLAQQASLAQQAELAQ Secondary structure E Vp] Guinea p i g Q H BAQEQFHRIN Rat K H H P R LS Q V Y Y P N M E Q P Y R K N A Y S Q V Q B R H P B S V Cow P Q L E 11Vl P N S A E E R L H S H S E G I H A O Q K E P B I G Secondary structure Guinea p i g Rat Cow a a a a a E H N - Q A Q V K - E P M R V F VD Q A Q r S V Q S F P Q L V N Q E L A Y F Y P E L F R Q F N Q L D S O Y G Y 0 L D Secondary structure 1« ISM D» Guinea pig R>t A|W|Y|Y|G|P|E ^ » 1 Y [ Y 1 G [ P 1 E - v|Q V | QYY | M S F L P F E S I P O | P I | F P E D A Q NITIE L HH LL VY FF P PO0 D B HQ Y Y L T P E B V L H T F IT P I A P K D A E N T N cow A|H|Y|Y|V|P|L H Y Y V P L G G T|Q T Q Y Y T D A P S F S D I P N P 1 0 S E N S E K T T Secondary structure Guinea pig Rat cow Hp EHv n N n p L|M| Secondary structure Fig. 4. Primary structures of a,|-caseins from the cow, rat and guinea pig, aligned according to Hall et ai. (1984b), Hobbs and Rosen (1982) and Merrier et al. (1985) with minor modifications. For symbols see Figure 1. 393  (...truncated)