IgG, IgM and IgA antibodies to GP43 (glycoprotein fraction of Paracoccidioides brasiliensis) were measured by ELISA in 63 samples from 23 patients with paracoccidioidomycosis before and twice after chemotherapy was started. Antibodies against P. brasiliensis were detected by indirect immunofluorescence (IF) (IgG, IgM and IgA isotypes), counterimmunoelectrophoresis (CIE) and...
The echinocandins and pneumocandins are lipopeptide antifungal agents that inhibit the synthesis of 1,3-β-d-glucan, an essential cell wall homopolysaccharide found in many pathogenic fungi. Compounds with this fungal-specific target have several attractive features: lack of mechanism-based toxicity, potential for fungicidal activity and activity against strains with intrinsic or...
Michael R. McGinnis; Chief editor's report, Journal of Medical and Veterinary Mycology, Volume 35, Issue 2, 1 March 1997, Pages 77, https://doi.org/10.1080
The glycoprotein gp43 from Paracoccidioides brasiliensis is the main antigenic component in paracoccidioidomycosis (PCM) because it is recognized by 100% of PCM patients. It has also been shown that different fungal strains produce gp43 with at least four isoform profiles. In this study, different isoform profiles from gp43, with pIs ranging from 5·8 to 8·5, were affinity...
The ultrastructural pattern of human hair infection by Piedraia hortae in vivo has been studied. The fungus destroyed the cuticular layers of the hair and was able to penetrate deeply into the cortex. The course of destruction of human hair is described and compared with that produced by other fungi. Two well-defined types of cortex digestion were characterized. The slow rate of...
Mice treated with cyclophosphamide were infected intravenously with titrated doses of Trichosporon asahii TIMM3140 or TIMM3144. Mortality and survival time correlated with inoculum size and fungal growth was found in the lung, heart, liver, spleen, kidney and brain. Fluconazole and amphotericin B prolonged survival time and reduced the CFU in the kidney, with fluconazole being...
Repeated DNA sequences were used to fingerprint strains of Aspergillus fumigatus isolated from a cow with disseminated systemic aspergillosis, cows with single aspergillosis lesions, calves aborted due to bovine aspergillosis, mothers of those calves, and cattle without aspergillosis. The analysis of the Southern blot hybridization patterns obtained suggested that: (i) the portal...
We assayed the fungicidal effects of antifungal agents using neutral red staining. Fungal elements of Trichophyton mentagrophytes and T. rubrum were treated with various concentrations of antifungal agents in 96-well filtration plates and then stained with neutral red. The amount of neutral red incorporated by the surviving viable cells was determined from the automated...
One hundred and fifty-nine isolates representing 16 genera and 53 species of yeasts were processed with the Biolog MicroStation System for yeast identification. Thirteen genera and 38 species were included in the Biolog database. For these 129 isolates, correct identifications to the species level were 13·2, 39·5 and 48·8% after 24, 48 and 72 hours incubation at 30 °C...
The aetiology of recurrent vulvovaginal candidiasis (RVVC) caused by Candida albicans remains unclear. To adequately address the role of antifungal resistance as a potential mechanism for RVVC, a longitudinal susceptibility analysis of 177 C. albicans isolates collected from 50 C. albicans RVVC patients over a period of 3 months to 7 years was performed. Antifungals tested...
The susceptibility of severe combined immune deficiency (SCID) mice to experimental systemic cryptococcosis was studied. SCID mice were at least 16-fold more susceptible to lethal infection than were immunocompetent control mice (P < 0·001). Histological assessment showed that control mice responded with a granulomatous inflammatory response, whereas SCID mice showed no evidence...
Circulating immune complexes (CIC) from 15 paracoccidioidomycosis (PCM) patient sera and from 20 healthy control sera were analysed. After CIC precipitation, solubilization and acid treatment, only a little reactivity to P. brasiliensis antigens was found in the free antibodies from PCM-CIC. This result has suggested that there were antibodies with a high affinity bound to fungus...
Because histoplasmosis is a life-threatening disease in AIDS and other compromised patients, we examined the efficacy of D0870 (Zeneca) in immunosuppressed mice against systemic histoplasmosis. Oral therapy with fluconazole given once daily (QD) was ineffective in prolonging survival, whereas itraconazole given once or twice daily (BID), fluconazole given BID or D0870 given QD or...
Two hundred and seventy-one healthy students and workers and 28 hospitalized tuberculosis patients living in Sichuan Province (southwest People's Republic of China; PRC) were tested with histolyn and tuberculin. The age of the tested subjects ranged from 17 to 22 years among the students, 17 to 38, among the workers and 17 to their 70s among the patients. Thirty-eight students...
Variations in molecular karyotype and fluconazole susceptibility of serial yeast isolates from the oral cavities of nine patients with AIDS receiving fluconazole for single or multiple episodes of oropharyngeal candidiasis were monitored. Multiple yeast species were isolated from the initial oral specimens in six patients. Molecular karyotyping identified at least eight different...
A reproducible model of a generalized Candida albicans infection was established in rats to allow a precise evaluation of the efficacy of antifungal compounds. In contrast to the intravenous C. albicans model in mice, which serves as a primary model for in vivo efficacy studies of antimycotic compounds, the infectious process in Sprague-Dawley rats is more severely spread into...
A T-T hybridoma (F6.6.2) which secretes a T-suppressor factor (TsF) specific for cryptococcal capsular polysaccharide (glucuronoxylomannan, GXM) was tested to determine if antigen-presenting cells (APC) were necessary for activation of the hybridoma to secrete TsF. Normal, syngeneic spleen cells were required along with GXM before TsF could be detected in culture supernatants. Ts...
Interactions between alveolar macrophages (AM) from rats and a yeast with relatively high pathogenicity (Candida albicans), a yeast with low pathogenicity (Saccharomyces cerevisiae) and an inert control particle (amorphous silica) of similar diameters, 3–4 μm, were studied. Both yeasts were phagocytized significantly faster by AM than were the control particles and C. albicans...
R. C. Matthews; Comparative assessment of the detection of candidal antigens as a diagnostic tool, Journal of Medical and Veterinary Mycology, Volume 34, I
P.H. Jacobs, D.J. Drutz, U. Budimulja, G. Cauwenbergh, Y. Koltin, S. Nolting, P. De Doncker; Progress in antifungal chemotherapy, Journal of Medical and Ve