We compared the ability of four commercially available yeast identification systems for routine laboratory hospital use: Auxacolor® (AUX) (Sanofi Diagnostics Pasteur, Marne-la-Coquette), Fungichrom® I (FUC) and Fungifast® ITwin (FUF) (International Microbio, Toulon), Api Candida® (API) (bio Mérieux, Lyon). These systems are based on obtaining a biochemical profile easily defined...
Systemic infection by an unclassified yeast-like organism has been encountered sporadically in wild-caught owl monkeys (Aotus sp.) from SouthAmerica. The infection is presumably acquired in the wild; the incubation period ranges from months to years. The disease is indolent and clinical signs are non-specific. The diagnosis is based on histopathologic observation of yeast-like...
The internal transcribed spacer (ITS) region, covering the ITS1, ITS2 and 5·8S ribosomal DNA was used to evaluate phylogenetic relationships within the fungal family Arthrodermataceae. Sequences of variable length, ranging between 522 and 684 base pairs were aligned. An unrooted consensus tree based on parsimony analysis showed Trichophyton to be polyphyletic, and Microsporum to...
Glucocorticoids are often recommended along with oral antifungals in the treatment of kerion celsi. In this randomized study, the efficacy of combination therapy with oral griseofulvin and oral prednisolone (n = 17) was compared to oral griseofulvin alone (n = 13) in the treatment of kerion celsi. Both groups were treated with oral griseofulvin for 8 weeks whereas oral...
We studied fungemia over time in outbred mice infected with Cryptococcus neoformans and looked at its relationship with the intravenous (i.v.) inoculum size, tissue burden and survival. Fungemia was evaluated by culture of 10 µl of peripheral blood from living mice or by culture of buffy coats from sacrificed animals. For all inoculum sizes studied, fungemia could last several...
M. J. Dykstra, N. J. H. Sharp, T. Olivry, A. Hillier, K. M. Murphy, L. Kaufman, G. A. Kunkle, C. Pucheu-Haston; A description of cutaneous-subcutaneous pyt
F. H. J. Van Enckevort, M. G. Netea, A. R. M. M. Hermus, C. G. J. Sweep, J. F. G. M. Meis, J. W. M. Van Der Meer, Bart Jan Kullberg; Increased susceptibili
B. Christensson, G. Sigmundsdottir, L. Larsson; D-arabinitol — a marker for invasive candidiasis, Medical Mycology, Volume 37, Issue 6, 1 January 1999, Pag
A. J. Hamilton, M. D. Holdom; Antioxidant systems in the pathogenic fungi of man and their role in virulence, Medical Mycology, Volume 37, Issue 6, 1 Janua
J. Guarro; Comments on recent human infections caused by ascomycetes, Medical Mycology, Volume 36, Issue 5, 1 January 1998, Pages 349, https://doi.org/10.1
We experienced an atypical case of pityriasis versicolor with a unique clinical appearance and undescribed mycological features. Although Malassezia sp. was cultured from the keratotic material, the fungal elements observed in the material were not readily identified as Malassezia. The diagnosis was established with the aid of immunohistochemical and ultrastructural studies with...
Filamentous fungi have a sturdy cell wall which is resistant to the usual DNA extraction procedures. We determined the DNA extraction procedure with the greatest yield of high quality fungal DNA and the leastpredilection for cross-contamination of equipment between specimens. Each of six extraction methods was performed using Aspergillus fumigatus hyphae. The six methods were:(1...
In order to speed up and standardize polymerase chain reaction (PCR)-based detection of medically important fungi in clinical samples we established a combination of commercially available kits for DNA extraction, PCR amplification and detection of the amplicons. The PCR plate assay proved to be as sensitive and specific as our previously published assay (5 cfu ml−1 blood...
Voriconazole is a new triazole antifungal agent with potent activity against yeast and moulds. We investigated the in vitro activity of voriconazole, itraconazole, amphotericin B and 5-flucytosine against 51 clinical isolates of filamentous fungi. Overall, voriconazole was active (MIC50, 0·5 mgl-1 and MIC90, 8 mgl-1) against these mould isolates. Voriconazole was most active...
Adiaspores of Emmonsia crescens were foundin the lungs of 62·1% of 87 adult rodents from 10 windbreaks compared to only 8·2% of 184 adult rodents caught in 10 adjacent arable fields in South Moravia, Czechland. A significantly higher mean weight proportion of plant remnants (predominantly small roots) was present in the soil from windbreaks (0·74%) than from fields (0·24%). In...
Effective serodiagnosis of systemic fungal infections is of increasing importance, particularly with regard to the identification of infection with Histoplasma capsulatum, Paracoccidioides brasiliensis and Penicillium marneffei. Methodology has been based either around antibody or antigen detection, although there is clear overlap between the two. Antibody-based detection systems...
In 1991, 1208 cases of coccidioidomycosis were reported to the California Department of Health Services, compared with an annual average of 450 during 1986–90. We conducted a study in Tulare County to define the epidemiology of the disease and identify risk factors for severe disease, focusing on the epidemic period September 1991–December 1991. To identify cases, we used data...
Paranasal sinus mycoses are endemic in rural populations of northern India. To study host-parasite interactions, we developed an animal model of paranasal sinus mycoses. After failure in small animals such as mice and rats, we used New Zealand white rabbits weighing 2·5–3 kg. Inoculum sizes consisted of 0·75–1·0 × 108, 0·75–1·0 × 107, 0·75–1·0 × 106 conidia of a clinical isolate...
Contact-sensing or thigmotropism is the directional growth response of cells in relation to topographical guidance cues. Thigmotropism is thought to play a major role in the location of infectable sites on plants by phytopathogenic fungi and has recently been shown to be a property of hyphae in the human pathogenic fungus Candida albicans. Here we show that hyphae of the...
Cryptococcal meningitis was induced in mice using intracerebral injection of Cryptococcus neoformans. Beginning either 3 days before or 1 day after infection, mice were treated with human recombinant granulocyte colony stimulating factor (hGCSF). In high doses hGCSF reduced the brain tissue burden of C. neoformans but had no effect on survival. The effect of hGCSF was dependent...
Determinations of plasma cholesterol, HDL-cholesterol, triglycerides, total proteins, bilirubin, aspartate transaminases (AST), glucose, iron and total iron binding capacity were evaluated after 15 and 90 days of intraperitoneal infection with 5 × 106 virulent yeasts of Paracoccidioides brasiliensis Pb 18 isolate in resistant (A/J) and susceptible (B10.A) mice. Significant...
Two atopic siblings presented with unilateral chronic scaly plantar lesions. Histology revealed the presence of fungi with unusual morphological aspects. Scopulariopsis brevicaulis and Phoma spp., respectively, grew on repeated cultures. Dermatophytes were absent. Such opportunistic fungal infections of the stratum corneum are extremely rare.