The composition of a lyophobic phenolic preparation of propolis

THE CO~80SITION OF A LYOPHOBIC PHENOLIC PREPARATION OF PROPOLIS A. I. Tikhonov, V. I. Litvinenko, and L. I. Dranik UDC 547.972 The isolation of biologically active substances from propolis followed by the chemical investigation and elucidation of the interconnection between their structure and medical effect is an urgent question, particularly from the point of view of the standardization of this source of medicinal raw material [1-3]. By a method of fractlonal-differential extraction that we have developed, from forty European and Asian examples of propolis we have obtained the total lyophobic (ethanol-soluble) phenolic fractions. The individual substances were isolated by preparative paper chromatography followed by purification on polyamide sorbent. Five phenolic compounds were identified: apigenin, luteolin, kaempferol, quercetin, and rhabidanol. The substances mentioned are components of all the preparations isolated and, of course, of the initial propolis raw material. LITERATURE CITED i. 2. 3. S.A. Polravko, A. I. Gurevlch, and M. N. Kolosov, Khim. Prirodn. Soedln., 476 (1969). P. E. Keller and E. K. Prudnichenko, Abstracts of Lectures at the Second Leningrad Scientific Conference on the Use of Apicultural Products in Human and Veterinary Medicine [in Russian], Leningrad (1960), p. 53. A. I. Tikhonov and N. N. Kruglitskil, Farmatslya, 33 (1975). Zaporozh'e Medical Institute. Kharkov Scientiflc-Research Institute of Pharmaceutical Chemistry. Translated from Khlmiya Prirodnykh Soedlnenll, No. 6, p. 864, November-December, 1977. Original article submitted September 21, 1977. 0009-3130/77/1306-0731507.50 © 1978 Plenum Publishing Corporation 731  (...truncated)