Correction: Guangxi cobra venom-derived NGF promotes the osteogenic and therapeutic effects of porous BCP ceramic
Jin et al. Experimental & Molecular Medicine (2020) 52:181–182
https://doi.org/10.1038/s12276-020-0378-0
Experimental & Molecular Medicine
CORRECTION
Open Access
Correction: Guangxi cobra venom-derived NGF
promotes the osteogenic and therapeutic effects
of porous BCP ceramic
Pan Jin, Fuqiang Yin, Li Huang, Li Zheng, Jinmin Zhao and Xingdong Zhang
Correction to: Experimental & Molecular Medicine
https://doi.org/10.1038/emm.2016.173
published online 7 April 2017
Published online: 27 January 2020
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After online publication of this article, the authors noticed
an error in the Figure section. The correct statement of
this article should have read as below.
In the article cited above, incorrect figure was placed in
Fig. 2C-c. The corrected image of Fig. 2 is printed below.
Other parts of this article remain unchanged.
The authors apologize for any inconvenience caused.
© The Author(s) 2020
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Official journal of the Korean Society for Biochemistry and Molecular Biology
Jin et al. Experimental & Molecular Medicine (2020) 52:181–182
182
Fig. 2 Concentration screening, cytotoxicity assay, FDA-PI staining, rhodamine phalloidin-Hoechst 33258 staining, alkaline phosphatase
(ALP) activity assay, ALP staining, osteogenic-specific gene expression and Alizarin red staining of monolayer-cultured osteoblasts. A
Screening of nerve growth factor (NGF) using various concentrations (0.625, 1.25, 2.5, 5, 7.8125, 10, 15.625, 20, 31.25, 62.5, 125, 250 μg ml−1) on 2D
cultured osteoblasts using the MTT method after 3 days of treatment (n = 3). B Cytotoxicity assay with NGF at 0, 2.5, 5 and 10 μg ml−1 on days 2, 5
and 8 (n = 9). C (a–d) FDA-PI staining of osteoblasts treated with NGF at 0, 2.5, 5 and 10 μg ml−1 after 8 days of treatment (Scale bar = 200 μm). C
(e–h) Rhodamine phalloidin- Hoechst 33258 staining of a monolayer culture treated with 0, 2.5, 5 and 10 μg ml−1 NGF after 8 days (Scale bar =
100 μm). D ALP activity assay of osteoblasts treated with 0, 2.5, 5 and 10 μg ml−1 NGF on days 2, 5 and 8 (n = 9). E (a–d) ALP staining of osteoblasts
treated with 0, 2.5, 5 and 10 μg ml−1 NGF after 8 days (Scale bar = 500 μm). F (a–f) Relative expression of bone morphogenetic protein-2 (BMP2, F (a)),
runt-related transcription factor 2 (RUNX2, F (b)), alkaline phosphatase (ALP, F (c)), bone sialoprotein (BSP, F (d)), osteocalcin (OCN, F (e)) and alpha-1
type I collagen (COL1A1, F (f)) in osteoblasts treated with 0, 2.5, 5 and 10 μg ml−1 NGF on days 2, 5 and 8 (n = 3). G (a–d) Alizarin red staining of
osteoblasts treated with 0, 2.5, 5 and 10 μg ml−1 NGF after 8 days (Scale bar = 500 μm). The values are presented as the means ± standard deviation.
Bars with different letters at the same time point are significantly different from each other at P < 0.05, and bars with the same letter exhibit no
significant difference.
Official journal of the Korean Society for Biochemistry and Molecular Biology
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