ISOLATION AND IDENTIFICATION OF SOME PHYTOCHEMICAL COMPOUNDS FROM DIFFERENT PARTS OF IRIS NIGRICANS

European Scientific Journal February 2013 edition vol.9 ISOLATION AND IDENTIFICATION OF SOME PHYTOCHEMICAL COMPOUNDS FROM DIFFERENT PARTS OF IRIS NIGRICANS Sajee' Huwaitat Ekbal Al-Khateeb Samira Finjan 0 2 0 Footnotes: MS= Mass Spectrometry 1 H-NMR= Proton Nuclear Magnetic Resonance 2 Pharmaceutical Pharmacy department, Faculty of Pharmacy & Medical Sciences, Al-Ahliyya Amman University , Jordan 3 C- NMR= Carbon 13 Nuclear Magnetic Resonance Iris genus (Iridaceae) is an elegant endemic plant species, and considered threatened to Jordan due to habitat degradation. Rhizomes of some Iris species contain flavonoids such as iridin, iriline A, irisone B. Iris leaves contain many flavonoids such as irilin, iristectorigenins, tectorigenin, and coumaronochromones. Iris flowers contain also flavonoids and proanthocyanidin while Iris nigricans have no previous studies conducted on leaves and flowers. In this study some phytochemical compounds were separated from different parts of Iris nigricans (rhizomes, leaves and flowers) by methanol. Isolation is carried out by using chromatographic technique and identified by ferric chloride reagent and UV light max254365nm. The phenolic composition of methanolic extracts of Iris nigricans obtained from rhizomes, leaves and flowers were elucidated by MS, 1H-NMR and 13C-NMR. Methanolic extracts of Iris nigricans (different parts); chromatographic technique; MS; 1H-NMR; 13C-NMR; mangeferin; iristectoreginin A; proanthocyanidin - 9 10 2 3 Iristectorigenin A (C17H14O7) 8 A 5 2 3 OH O 1 F 4 OH 3' B 6' 2 3 Conclusion Since there is no previous studies on the chemical constituents of the leaves and References: Das DK, Sato M, and Ray PS, Cardioprotection of red wine: role of polyphenolic antioxidants, Drugs Exp Clin Res, 1999, 25:115-20. David G. Watson, pharmaceutical analysis, 2001, Pages 149-170. HOOH E. Nicole Bridgers , Mari S. Chinn and Van-Den Truong, Extraction of anthocyanins from industrial purple-fleshed sweetpotatoes and enzymatic hydrolysis of residues for fermentable sugars , Industrial Crops and Products , 2010 , Volume 32 , Pages 613 - 620 . Eva Miadokova ', Isoflavonoids - an overview of their biological activities and potential health benefits , InterdiscToxicol.; 2009 , Volume 2 , No. 4 , Pages 211 - 218 . Bull. 2001 , Volume 49 , No. 5 , Pages 551 - 557 . Le Minh Ha , Pan Van Kiem and Natalya Khripach , Chemical constituents of Belamcanda Chinensis (L.) DC, Journal of Chemistry , 2009 , Volume 47 , No. 5 , Pages 623 - 627 . Nathalie Wauthoz , Aliou Balde , Elhadj Sadou Balde, Marc Van Damme and Pierre Duez , Ethnopharmacology of Mangifera indica L. bark and pharmacological studies of its main CGlucosylxanthone , Mangiferin, International Journal of Biomedical and Pharmaceutical Sciences , 2007 , Pages 113 - 119 . Nighat Nazir , Surrinder Koul and Mushtaq Ahmad Qurishi, New isoflavones from Iris kashmiriana , Journal of Asian Natural Products Research , 2008 , Volume 10 , No. 12 , Pages 1137 - 1141 . Rana Abu-Dahab , and Fatma Afifi , Antiproliferative activity of selected medicinal plants of Jordan against a breast adenocarcinoma cell line (MCF7), Scientia Pharmaceutica , 2007 , Volume75, Pages121 - 136 . Ranju Pal , Kundlik Girhepunje , Ashutosh Upadhayay and Thirumoorthy N. , Antioxidant and free radical scavenging activity of ethanolic extract of the root of Morinda citrifolia (Rubiaceae) , African Journal of Pharmacy and Pharmacology , 2012 , Volume 6 , No. 5 , Pages 278 - 282 . Sajee' Huwaitat, Ekbal AL-khateeb, Samira Finjan and Anwar Maraqa, Antioxidant and antimicrobial activities of Iris nigricans methanolic extracts containing phenolic compounds , European Scientific Journal , 2013 , Volume 9 , No. 1 , Pages 1 - 10 . Syeda F. Asghar , Shahid Aziz and Habib-ur-Rehman, Secondary Metabolites Isolated from Iris germanica , Records of Natural Products , 2009 , Volume 3 , No. 3 , Pages139 - 152 . V. H. Booth and B. J. Constable , Stability of ascorbic acid in extracts of Iris leaves , Biochemical Journal , 1960 , Volume76, Page 206. (...truncated)