Correction: Atorvastatin Improves Survival in Septic Rats: Effect on Tissue Inflammatory Pathway and on Insulin Signaling

March Correction: Atorvastatin Improves Survival in Septic Rats: Effect on Tissue Inflammatory Pathway and on Insulin Signaling The PLOS ONE Staff OPEN ACCESS - Fig 2. Effects of atorvastatin treatment on insulin signaling in the CLP rat. Representative blots show insulin-induced tyrosine phosphorylation of Insulin Receptor (IR) in liver (A), muscle (B) and adipose (C) of sham and septic rats. Total protein expression of IR (AC, lower panels). Insulin-induced tyrosine phosphorylation of Insulin Receptor Substrate 1 (IRS1) in liver (D), muscle (E) and adipose tissue (F) of sham and septic rats. Total protein expression of IRS1 (DF, lower panels). Insulin-induced serine phosphorylation of Akt in liver (G), muscle (H) and adipose (I) of sham and septic rats. Insulininduced threonine phosphorylation and total protein expression of Akt (GI, lower panels). In this case, blots were stripped and reprobed with -actin (AI, lower panels) to confirm equal loading of proteins. Data are presented as means +/ S.E.M from 68 rats per group. *P<0.05 (Sepsis/Sal vs. all others groups). IB, immunoblot; CLT: Sham/Saline; ShT: Sham/Atorvastatin; SAL: saline; ATOR: atorvastatin. Fig 4. Representative blots show the JNK phosphorylation in liver (A), muscle (B) and adipose tissue (C) of sham and septic rats (upper panels). Total protein expression of JNK (AC, lower panels). Phosphorylation of c-jun in liver (D), muscle (E) and adipose tissue (F) of sham and septic rats. Serine 307 Phosphorylation of IRS1 in liver (G), muscle (H) and adipose tissue (I) of sham and septic rats (upper panels). Total protein expression of IRS-1 (GI, lower panels). Data are presented as means S.E.M from 68 rats per group. *P<0.05 (Sepsis/Sal vs. all others groups); **P<0.001 (Sepsis/Sal vs. control); #P<0.05 (Sepsis/Sal vs. Sepsis/Ator). IB, immunoblot; CLT: Sham/Saline; ShT: Sham/Atorvastatin; SAL: saline; ATOR: atorvastatin. Fig 5. Representative blots show the NFkB activation in nuclear fractions of liver (A), muscle (B) and adipose tissue (C) of sham and septic rats. In this case blots were stripped and reprobed with actin (AC, lower panels) to confirm equal loading of proteins. Tissue levels of iNOS (DF) and IL-6 (GI) expression in liver, muscle and adipose tissue of sham and septic rats. Data are presented as means S.E.M from 68 rats per group. *P<0.05 (Sepsis/Sal vs. all others groups); **P<0.001 (Sepsis/Sal vs. control); #P<0.05 (Sepsis/Sal vs. Sepsis/Ator). IB, immunoblot; CLT: Sham/Saline; ShT: Sham/ Atorvastatin; SAL: saline; ATOR: atorvastatin. Fig 6. Representative blots show the PERK phosphorylation in liver (A), muscle (B) and adipose tissue (C) of sham and septic rats. eIF2 phosphorylation (DF) and ATF6 (GI) expression in liver, muscle and adipose tissue of sham and septic rats. In this case, blots were stripped and reprobed with actin (AI, lower panels) to confirm equal loading of proteins. Data are presented as means S.E.M from 68 rats per group. *P<0.05 (Sepsis/Sal vs. all others groups); #P<0.05 (Sepsis/Sal vs. Sepsis/Ator). IB, immunoblot; CLT: Sham/Saline; ShT: Sham/Atorvastatin; SAL: saline; ATOR: atorvastatin. Supporting Information 1. Calisto KL , Carvalho BdM , Ropelle ER , Mittestainer FC , Camacho ACA , Guadagnini Dioze , et al. ( 2010 ) Atorvastatin Improves Survival in Septic Rats: Effect on Tissue Inflammatory Pathway and on Insulin Signaling . PLoS ONE 5(12): e14232. doi: 10.1371/journal.pone.0014232 PMID: 21151908 (...truncated)