Preparation of an ochre suppressor tRNA recognizing exclusively UAA codon by using the molecular surgery technique

Nucleic Acids Symposium Series, Sep 2009

In order to create an ochre suppressor tRNA which exclusively recognizes UAA codon, we replaced the G34 at the first position of yeast tRNATyr[GΨA] anticodon with pseudouridine34 (Ψ34) by using the molecular surgery technique. This tRNATyr[ΨΨA] recognized only the UAA codon as expectedly, but tRNATyr[UΨA] made as a control also behaved similarly. This result may suggest that U34 must be somehow modified to facilitate the wobble-pairing to G at the third position of codon.

Article PDF cannot be displayed. You can download it here:

https://academic.oup.com/nass/article-pdf/53/1/295/3841050/nrp148.pdf

Preparation of an ochre suppressor tRNA recognizing exclusively UAA codon by using the molecular surgery technique

ß 2009 Oxford University Press Symposium date: 27th September 2009 Nucleic Acids Symposium Series No. 53 295 doi:10.1093/nass/nrp148 296 Nucleic Acids Symposium Series No. 53 (...truncated)


This is a preview of a remote PDF: https://academic.oup.com/nass/article-pdf/53/1/295/3841050/nrp148.pdf
Article home page: https://academic.oup.com/nass/article/53/1/295/1099737

Yokogawa, Takashi, Hassan, Hanim Munirah Binti Che, Yokota, Yukiko, Ohno, Satoshi, Nishikawa, Kazuya. Preparation of an ochre suppressor tRNA recognizing exclusively UAA codon by using the molecular surgery technique, Nucleic Acids Symposium Series, 2009, pp. 295-296, Volume 53, Issue 1, DOI: 10.1093/nass/nrp148